2026-04-25T23:23:50Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/353302025-03-26T19:10:03Zcom_10324_22821com_10324_954com_10324_894col_10324_22822

Flora, Tatjana González de Torre, Israel Quintanilla Sierra, Luis Alonso Rodrigo, Matilde Rodríguez Cabello, José Carlos 2018 Producción Científica A simple system for cell selectivity and spatially controlled cell adhesion has been developed using model gold surfaces grafted with a combination of two ELRs containing into their backbone cell-adhesion domains such as RGD and REDV. Grafting onto gold was achieved via redox reaction through thiol groups present in amino terminal cysteine tails of the ELRs. The correlation among contact angle, SEM micrographs, AFM, XPS and QCM-D have been carried out. After in-depth adhesion studies, a mixture of 75% ELR-REDV and 25% ELR-RGD was found to exhibit high selectivity for endothelial cells, promoting strong adhesion thereof. Consequently, certain areas of gold surfaces (strips) were cleaned by laser ablation and functionalized with the mixture 75% ELR-REDV - 25% ELR-RGD leading to a spatial segregation of the co-culture made of HUVEC and HFF1 cells. This platform therefore exhibits selective spatial control over cell adhesion associated with the bioactive epitopes (RGD and REDV) contained in the ELR sequence, since each functionalized surface (including strips) have similar topographic, hydrophobic and mechanical properties. application/pdf http://uvadoc.uva.es/handle/10324/35330 eng Elsevier Spatial control and cell adhesion selectivity on model gold surfaces grafted with elastin-like recombinamers info:eu-repo/semantics/article TEXT UVaDOC. Repositorio Documental de la Universidad de Valladolid Hispana