2026-05-05T19:34:26Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/408442024-12-17T13:15:27Zcom_10324_1183com_10324_931com_10324_894col_10324_1396

Effect of mycoviruses on growth, spore germination and pathogenicity of the fungus Fusarium circinatum Flores Pacheco, Juan Asdrúbal Muñoz Adalia, Emigdio Jordan Martínez Álvarez, Pablo Pando Fernández, Valentín Díez Casero, Julio Javier Martín García, Jorge Pine pitch canker Chancro resinoso del pino Hypovirulence Hipovirulencia Biological control Control biológico Forest pathology Patología forestal 3106 Ciencia Forestal Producción Científica Aim of the study: To assess the impact on two mycoviruses recently described in F. circinatum mitovirus 1, and 2-2 (FcMV1 and FcMV2-2) on i) mycelial growth, ii) spore germination and iii) relative necrosis. Material and methods: Fourteen monosporic strains of F. circinatum (one of each pair infected with mycoviruses and the other without them) of the pathogen with and without viruses were selected for the assay. The statistical analysis, were a linear mixed model of analysis of variance considering one between-subjects factor (isolate) and one within-subjects factor with four levels (1=without viruses, 2=only virus FcMV1, 3=only virus FcMV2-2 and 4=both viruses). Main results: Colony growth rates of F. circinatum isolates were significantly reduced in presence of mycoviruses (p=0.002). The spore germination was also reduced in the F. circinatum isolates containing mycovirus as compared to mycovirus-free isolates (p<0.001). No significant differences in lesion lengths caused by F. circinatum were found in relation to the presence/absence of mycovirus (p<0.61). Research highlights: Reduction of the percentage of spore germination in the isolates of F. circinatum with mycovirus infections, as compared to free isolates, provides indications of reduction of metabolic activity and plant physiology are discussed. The lack of significant differences found in the length of the lesions caused by F. circinatum with respect to the presence/absence of mycovirus, indicates that further studies with a larger number of variables are required. Ministerio de Economía, Industria y Competitividad (projects AGL2012-39912 and AGL2015- 69370-R) European Cooperation in Science and Technology (COST Action FP1406 PINESTRENGTH) Portuguese Foundation for Science and Technology (grant SFRH/BPD/122928/2016) 2020-05-14T11:30:52Z 2020-05-14T11:30:52Z 2017 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Forest systems, 2017, vol. 26, n. 3. 7 p. 2171-9845 http://uvadoc.uva.es/handle/10324/40844 10.5424/fs/2017263-11060 eng https://revistas.inia.es/index.php/fs/article/view/11060 Attribution-NonCommercial-NoDerivatives 4.0 Internacional info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ © 2017 Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria application/pdf Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) https://uvadoc.uva.es/bitstream/10324/40844/4/Effect-of-mycoviruses-on-growth.pdf.jpg Hispana TEXT http://creativecommons.org/licenses/by-nc-nd/4.0/ UVaDOC. Repositorio Documental de la Universidad de Valladolid http://uvadoc.uva.es/handle/10324/40844