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oai:uvadoc.uva.es:10324/409972021-06-23T11:44:41Zcom_10324_1181com_10324_931com_10324_894col_10324_1387

Iglesias Álvarez, María del Rosario Pérez, Yolanda Citores González, Lucía Ferreras Rodríguez, José Miguel Méndez, Enrique Girbés Juan, Tomás 2020-06-05T11:24:41Z 2020-06-05T11:24:41Z 2008 Journal of Experimental Botany, 2008, vol. 59, n. 6. p. 1215-1223 0022-0957 http://uvadoc.uva.es/handle/10324/40997 10.1093/jxb/ern030 Producción Científica BE27 and BE29 are two forms of beetin, a virus-inducible type 1 ribosome-inactivating protein isolated from leaves of Beta vulgaris L. Western blot analysis revealed the presence of beetin forms in adult plants but not in germ or young plants, indicating that the expression of these proteins is developmentally regulated. While beetins are expressed only in adult plants, their transcripts are present through all stages of development. In addition, the treatment of B. vulgaris leaves with mediators of plant-acquired resistance such as salicylic acid and hydrogen peroxide promoted the expression of beetin by induction of its transcript, but only in adult plants. The plant expresses three mRNAs which differ only in their 3′ untranslated region. All these observations suggest a dual regulation of beetin expression, i.e. at the post-transcriptional and transcriptional levels. Additionally, total RNA isolated from leaves treated with hydrogen peroxide, which express high levels of active beetin, is not de-adenylated by endogenous beetin, nor in vitro by the addition of BE27, thus suggesting that sugar beet ribosomes are resistant to beetin. Junta de Castilla y Leon (grant VA0150A7) application/pdf eng Oxford University Press info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/3.0/ © 2008 Oxford University Press Attribution-NonCommercial-NoDerivs 3.0 Unported Beet Remolacha Hydrogen peroxide Peróxido de hidrógeno Ribosome-inactivating proteins Proteínas inactivadoras de ribosomas Salicylic acid Acido salicílico Elicitor-dependent expression of the ribosome-inactivating protein beetin is developmentally regulated info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://academic.oup.com/jxb/article/59/6/1215/481750 SI