2026-05-05T19:48:08Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/409982021-06-23T11:44:43Zcom_10324_1181com_10324_931com_10324_894col_10324_1387

Girbés Juan, Tomás Barbieri, Luigi Ferreras Rodríguez, José Miguel Arias Vallejo, Francisco Javier Iglesias Álvarez, María del Rosario Alegre, Carlos Escarmis, Cristina Stirpe, Fiorenzo Producción Científica The effects of 30 type 1 and of 2 (ricin and volkensin) type 2 ribosome-inactivating proteins (RIPs) on Escherichia coli and Agrobacterium tumefaciens cell-free translation systems were compared with the effects on a rabbit reticulocyte translation system. The depurinating activity of RIPs on E. coli ribosomes was also evaluated. Only six type 1 RIPs inhibited endogenous mRNA-directed translational activity of E. coli lysates, with submicromolar 50% inhibitory concentrations. Four RIPs had similar activities on poly(U)-directed phenylalanine polymerization by E. coli ribosomes, and three RIPs inhibited poly(U)-directed polyphenylalanine synthesis by A. tumefaciens ribosomes, with submicromolar 50% inhibitory concentrations. 2020-06-05 2020-06-05 1993 info:eu-repo/semantics/article Journal of Bacteriology, 1993, vol. 175, n. 20. p. 6721-6724 0021-9193 http://uvadoc.uva.es/handle/10324/40998 10.1128/jb.175.20.6721-6724.1993 eng https://jb.asm.org/content/175/20/6721 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/3.0/ © 1993 American Society for Microbiology Attribution-NonCommercial-NoDerivs 3.0 Unported American Society for Microbiology