2026-05-05T18:46:22Zhttps://uvadoc.uva.es/oai/request
oai:uvadoc.uva.es:10324/411142022-01-10T13:53:05Zcom_10324_1181com_10324_931com_10324_894col_10324_1387
UVaDOC author Comino, Isabel author Fernández Bañares, Fernando author Esteve, María author Ortigosa, Luís author Castillejo, Gemma author Fambuena, Blanca author Ribes-Koninckx, Carmen author Sierra, Carlos author Rodríguez Herrera, Alfonso author Salazar, José Carlos author Caunedo, Ángel author Marugán de Miguelsanz, José Manuel author Garrote Adrados, José Antonio author Vivas, Santiago author lo Iacono, Oreste author Nuñez, Alejandro author Vaquero, Luis author Vegas Álvarez, Ana María author Crespo Valderrabano, Laura author Fernández Salazar, Luis Ignacio author Jiménez García, Victoria Alejandra author Montes Cano, Marco Antonio author Espín, Beatriz author Galera, Ana author Valverde, Justo author Girón, Francisco José author Bolonio, Miguel author Millán, Antonio author Martínez Cerezo, Francesc author Guajardo, César author Alberto, José Ramón author Rosinach, Mercé author Segura, Verónica author León, Francisco author Marinich, Jorge author Muñoz Suano, Alba author Romero Gómez, Manuel author Cebolla, Ángel author Sousa, Carolina author Arranz Sanz, Eduardo 2020-06-29T09:22:16Z 2020-06-29T09:22:16Z 2016 The American Journal of Gastroenterology, 2016,111:1456–1465 0002-9270 http://uvadoc.uva.es/handle/10324/41114 10.1038/ajg.2016.439 1456 10 1465 American Journal of Gastroenterology 111 Objectives: Treatment for celiac disease (CD) is a lifelong strict gluten-free diet (GFD). Patients should be followed-up with dietary interviews and serology as CD markers to ensure adherence to the diet. However, none of these methods offer an accurate measure of dietary compliance. Our aim was to evaluate the measurement of gluten immunogenic peptides (GIP) in stools as a marker of GFD adherence in CD patients and compare it with traditional methods of GFD monitoring. Methods: We performed a prospective, nonrandomized, multicenter study including 188 CD patients on GFD and 84 healthy controls. Subjects were given a dietary questionnaire and fecal GIP quantified by enzyme-linked immunosorbent assay (ELISA). Serological anti-tissue transglutaminase (anti-tTG) IgA and anti-deamidated gliadin peptide (anti-DGP) IgA antibodies were measured simultaneously. Results: Of the 188 celiac patients, 56 (29.8%) had detectable GIP levels in stools. There was significant association between age and GIP in stools that revealed increasing dietary transgressions with advancing age (39.2% in subjects ≥13 years old) and with gender in certain age groups (60% in men ≥13 years old). No association was found between fecal GIP and dietary questionnaire or anti-tTG antibodies. However, association was detected between GIP and anti-DGP antibodies, although 46 of the 53 GIP stool-positive patients were negative for anti-DGP. Conclusions: Detection of gluten peptides in stools reveals limitations of traditional methods for monitoring GFD in celiac patients. The GIP ELISA enables direct and quantitative assessment of gluten exposure early after ingestion and could aid in the diagnosis and clinical management of nonresponsive CD and refractory CD. Trial registration number NCT02711397 eng Attribution-NonCommercial-NoDerivatives 4.0 Internacional Fecal Gluten Peptides Reveal Limitations of Serological Tests and Food Questionnaires for Monitoring Gluten-Free Diet in Celiac Disease Patients info:eu-repo/semantics/article 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URL https://uvadoc.uva.es/bitstream/10324/41114/1/Fecal_Gluten_Peptides_Reveal_Limitations_of.24.pdf File MD5 a66b7f1c48e0107b9a9c7c90e6f03f33 381770 application/pdf Fecal_Gluten_Peptides_Reveal_Limitations_of.24.pdf