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Expression of MicroRNA-15b and the glycosyltransferase GCNT3 correlates with antitumor efficacy of rosemary diterpenes in colon and pancreatic cancer González-Vallinas Garrachón, Margarita Molina, Susana Vicente, Gonzalo Zarza, Virginia Martín Hernández, Roberto García Risco, Mónica R. Fornari, Tiziana Reglero, Guillermo Ramírez de Molina, Ana Cáncer de colon Cáncer de páncreas Romero 3207.13 Oncología Producción Científica Colorectal and pancreatic cancers remain important contributors to cancer mortality burden and, therefore, new therapeutic approaches are urgently needed. Rosemary (Rosmarinus officinalis L.) extracts and its components have been reported as natural potent antiproliferative agents against cancer cells. However, to potentially apply rosemary as a complementary approach for cancer therapy, additional information regarding the most effective composition, its antitumor effect in vivo and its main molecular mediators is still needed. In this work, five carnosic acid-rich supercritical rosemary extracts with different chemical compositions have been assayed for their antitumor activity both in vivo (in nude mice) and in vitro against colon and pancreatic cancer cells. We found that the antitumor effect of carnosic acid together with carnosol was higher than the sum of their effects separately, which supports the use of the rosemary extract as a whole. In addition, gene and microRNA expression analyses have been performed to ascertain its antitumor mechanism, revealing that up-regulation of the metabolic-related gene GCNT3 and down-regulation of its potential epigenetic modulator miR-15b correlate with the antitumor effect of rosemary. Moreover, plasmatic miR-15b down-regulation was detected after in vivo treatment with rosemary. Our results support the use of carnosic acid-rich rosemary extract as a complementary approach in colon and pancreatic cancer and indicate that GCNT3 expression may be involved in its antitumor mechanism and that miR-15b might be used as a non-invasive biomarker to monitor rosemary anticancer effect. 2020-09-28T07:22:38Z 2020-09-28T07:22:38Z 2014 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion PLoS ONE, 2014, vol 9, n. 6, e98556 1932-6203 http://uvadoc.uva.es/handle/10324/42590 10.1371/journal.pone.0098556 e98556 6 PLoS ONE 9 1932-6203 eng https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0098556 Attribution-NonCommercial-NoDerivatives 4.0 Internacional Attribution-NonCommercial-NoDerivatives 4.0 Internacional info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ © Public Library of Science application/pdf Public Library of Science https://uvadoc.uva.es/bitstream/10324/42590/4/Expression-of-MicroRNA-15b.PDF.jpg Hispana TEXT http://creativecommons.org/licenses/by-nc-nd/4.0/ UVaDOC. Repositorio Documental de la Universidad de Valladolid http://uvadoc.uva.es/handle/10324/42590