2026-04-14T17:02:51Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/433612021-06-24T07:27:57Zcom_10324_22821com_10324_954com_10324_894col_10324_22822

Acosta Rodríguez, Sergio Poócza, Leander Aaron Quintanilla Sierra, Luis Rodríguez Cabello, José Carlos 2020-11-06T10:08:50Z 2020-11-06T10:08:50Z 2020 Biomacromolecules 2020 (in press) 1525-7797 http://uvadoc.uva.es/handle/10324/43361 10.1021/acs.biomac.0c00934 Biomacromolecules 1526-4602 Intrinsically disordered protein polymers (IDPPs) have attracted a lot of attention in the development of bioengineered devices and for use as study models in molecular biology because of their biomechanical properties and stimuli-responsiveness. The present study aims to understand the effect of charge density on the self-assembly of IDPPs. To that end, a library of recombinant IDPPs based on an amphiphilic diblock design with different charge densities was bioproduced, and their supramolecular assembly was characterized on the nano-, meso-, and microscale. Although the phase transition was driven by the collapse of hydrophobic moieties, the hydrophilic block composition strongly affected hierarchical assembly and, therefore, enabled the production of new molecular architectures, thus leading to new dynamics that govern the liquid–gel transition. These results highlight the importance of electrostatic repulsion for the hierarchical assembly of IDPPs and provide insights into the manufacture of supramolecular protein-based materials. The authors are grateful for funding from the European Commission (MSCA-ITN-2014-ETN-642687), the Spanish Government (MAT2016-78903-R, MAT2015-68901-R and PCIN-2015-010 (FunBioPlas)), Junta de Castilla y León (VA317P18) and Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León. application/pdf spa ACS Publications info:eu-repo/semantics/openAccess Charge Density as a Molecular Modulator of Nanostructuration in Intrinsically Disordered Protein Polymers info:eu-repo/semantics/article info:eu-repo/semantics/draft https://pubs.acs.org/doi/10.1021/acs.biomac.0c00934 SI