2026-04-27T07:52:01Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/433612021-06-24T07:27:57Zcom_10324_22821com_10324_954com_10324_894col_10324_22822

00925njm 22002777a 4500 dc Acosta Rodríguez, Sergio author Poócza, Leander Aaron author Quintanilla Sierra, Luis author Rodríguez Cabello, José Carlos author 2020 Intrinsically disordered protein polymers (IDPPs) have attracted a lot of attention in the development of bioengineered devices and for use as study models in molecular biology because of their biomechanical properties and stimuli-responsiveness. The present study aims to understand the effect of charge density on the self-assembly of IDPPs. To that end, a library of recombinant IDPPs based on an amphiphilic diblock design with different charge densities was bioproduced, and their supramolecular assembly was characterized on the nano-, meso-, and microscale. Although the phase transition was driven by the collapse of hydrophobic moieties, the hydrophilic block composition strongly affected hierarchical assembly and, therefore, enabled the production of new molecular architectures, thus leading to new dynamics that govern the liquid–gel transition. These results highlight the importance of electrostatic repulsion for the hierarchical assembly of IDPPs and provide insights into the manufacture of supramolecular protein-based materials. Biomacromolecules 2020 (in press) 1525-7797 http://uvadoc.uva.es/handle/10324/43361 10.1021/acs.biomac.0c00934 Biomacromolecules 1526-4602 Charge Density as a Molecular Modulator of Nanostructuration in Intrinsically Disordered Protein Polymers