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oai:uvadoc.uva.es:10324/44432025-02-10T12:42:31Zcom_10324_1181com_10324_931com_10324_894col_10324_1387

Gato Casado, Ángel Luis Moro Balbás, José Antonio Pastor Vázquez, Juan Francisco Represa de la Guerra, Juan Barbosa Ayucar, Enrique 2014-03-03T11:03:24Z 2014-03-03T11:03:24Z 1993 Anat. Embryol. 0340-2061 http://uvadoc.uva.es/handle/10324/4443 101 188 106 Anat. Embryol. 188 Abstraer. Prcvious studies of thc car l y dcvclopment of the ncural tube ha ve shown thc ex istence of an intraneural nuid, which causes a positivc pressure inside this pri­ m ordium, and seems to play a key role in the early dcvcl­ opmcn l of the central nervous syslc m. I n the present sludy wc invest igated the composition and synthesis of lhis int ra neural fluid. By usi n g a scqucntial method, wh ich includes fixation with gl utaraldch yde plus cclylpyridinium chl oride, opening thc ncural cavity after critica! point drying and scan ning clcctron microscopy analysis, we found a water-soluble extracellular matrix that filled up thc brain vesicles of chick embryos al the ea rliest stages of thc ncural tu be. An ultrastructura l study of the neural cpit hcliu m during these stages revea led the cxistcnce of a secretion proccss in the neural cells toward thc apical side, the future neural cavity. An immunocyto­ chemical study to asscs thc nature of the secreted materi­ al has shown that the intraneu ral matrix conta ins chon­ droitin sulphate proteoglycan, which appeared homoge­ n ously distributed throughout lhc neura l cavity. Our findings demonstra te that thc int raneural liquid is a nuid of com plcx composilion and includes chondroiti n su l­ phate protcoglycan as a n osmotically active molccule. Th is suggests a morphogenetic role for the proteoglycan during ea rl y brain enlargcmcnt. Thc neural ectoderm is a polarized epi thelium from carly developmental stages and secretes the in t rancural malrix. spa info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ Attribution-NonCommercial-NoDerivatives 4.0 International Fluido intraneural - Composición Chondroitin sulphate proteoglycan and embryonic brain inlargement in the chick info:eu-repo/semantics/article