2026-04-23T21:22:01Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/450332021-06-24T07:16:34Zcom_10324_44585com_10324_954com_10324_894col_10324_44586

Hidalgo, Jorge Teuber, Stefanie Morera, Francisco J. Ojeda, Camila Flores, Carlos A. Hidalgo, María A. Núñez Llorente, Lucía Villalobos Jorge, Carlos Burgos, Rafael A. 2021-01-18T08:53:47Z 2021-01-18T08:53:47Z 2017 International Journal of Molecular Sciences, 2017, vol. 18, n. 4. 18 p. 1422-0067 http://uvadoc.uva.es/handle/10324/45033 10.3390/ijms18040750 Producción Científica Anthocyanins are pigments with antihyperglycemic properties, and they are potential candidates for developing functional foods for the therapy or prevention of Diabetes mellitus type 2 (DM2). The mechanism of these beneficial effects of anthocyanins are, however, hard to explain, given their very low bioavailability due to poor intestinal absorption. We propose that free fatty acid receptor 1 (FFA1, also named GPR40), is involved in an inhibitory effect of the anthocyanidin delphinidin over intestinal glucose absorption. We show the direct effects of delphinidin on the intestine using jejunum samples from RF/J mice, and the human intestinal cell lines HT-29, Caco-2, and NCM460. By the use of specific pharmacological antagonists, we determined that delphinidin inhibits glucose absorption in both mouse jejunum and a human enterocytic cell line in a FFA1-dependent manner. Delphinidin also affects the function of sodium-glucose cotransporter 1 (SGLT1). Intracellular signaling after FFA1 activation involved cAMP increase and cytosolic Ca2+ oscillations originated from intracellular Ca2+ stores and were followed by store-operated Ca2+ entry. Taken together, our results suggest a new GPR-40 mediated local mechanism of action for delphinidin over intestinal cells that may in part explain its antidiabetic effect. These findings are promising for the search for new prevention and pharmacological treatment strategies for DM2 management. INNOVA CORFO (grant 12IDL2-16254) Comisión Nacional de Investigación Científica y Tecnológica (grant 21130644) Ministerio de Economía, Industria y Competitividad (grant BFU2015-70131-R) application/pdf eng MDPI info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ © 2017 MDPI Attribution-NonCommercial-NoDerivatives 4.0 Internacional Delphinidin Delfinidina Anthocyanins Antocianina Glucose Glucosa Delphinidin reduces glucose uptake in mice jejunal tissue and human intestinal cells lines through FFA1/GPR40 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.mdpi.com/1422-0067/18/4/750 SI