2026-04-27T21:28:22Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/450672021-09-27T08:44:25Zcom_10324_44585com_10324_954com_10324_894col_10324_44586

Molina Guijarro, José Manuel Macías, Álvaro García Rodríguez, Carolina Muñoz Conejero, Eva García Fernández, Luis F. David, Miren Núñez Llorente, Lucía Martínez Leal, Juan Fernando Moneo, Victoria Cuevas, Carmen Lillo, María Pilar Villalobos Jorge, Carlos Valenzuela, Carmen Galmarini, Carlos María 2021-01-19T08:33:43Z 2021-01-19T08:33:43Z 2011 PLoS ONE, 2011, vol. 6, n. 4. 13 p. 1932-6203 http://uvadoc.uva.es/handle/10324/45067 10.1371/journal.pone.0019042 Producción Científica Irvalec is a marine-derived antitumor agent currently undergoing phase II clinical trials. In vitro, Irvalec induces a rapid loss of membrane integrity in tumor cells, accompanied of a significant Ca2+ influx, perturbations of membrane conductivity, severe swelling and the formation of giant membranous vesicles. All these effects are not observed in Irvalec-resistant cells, or are significantly delayed by pretreating the cells with Zn2+. Using fluorescent derivatives of Irvalec it was demonstrated that the compound rapidly interacts with the plasma membrane of tumor cells promoting lipid bilayer restructuration. Also, FRET experiments demonstrated that Irvalec molecules localize in the cell membrane close enough to each other as to suggest that the compound could self-organize, forming supramolecular structures that likely trigger cell death by necrosis through the disruption of membrane integrity. Ministerio de Ciencia, Innovación y Universidades (grants SAF2007-65868 , SAF2010-14916, FIS RD06/0014/0006, PIF200680F0023, S-BIO-0260/2006 and CTQ 2009-12412) application/pdf eng Public Library of Science info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/3.0/ © 2011 Public Library of Science Attribution-NonCommercial-NoDerivs 3.0 Unported Antitumor drugs Fármacos antitumorales Plasma membrane Membrana plasmática Irvalec inserts into the plasma membrane causing rapid loss of integrity and necrotic cell death in tumor cells info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0019042 SI