2026-05-05T11:36:51Zhttps://uvadoc.uva.es/oai/request
oai:uvadoc.uva.es:10324/450672021-09-27T08:44:25Zcom_10324_44585com_10324_954com_10324_894col_10324_44586
UVaDOC author Molina Guijarro, José Manuel author Macías, Álvaro author García Rodríguez, Carolina author Muñoz Conejero, Eva author García Fernández, Luis F. author David, Miren author Núñez Llorente, Lucía author Martínez Leal, Juan Fernando author Moneo, Victoria author Cuevas, Carmen author Lillo, María Pilar author Villalobos Jorge, Carlos author Valenzuela, Carmen author Galmarini, Carlos María 2021-01-19T08:33:43Z 2021-01-19T08:33:43Z 2011 PLoS ONE, 2011, vol. 6, n. 4. 13 p. 1932-6203 http://uvadoc.uva.es/handle/10324/45067 10.1371/journal.pone.0019042 Irvalec is a marine-derived antitumor agent currently undergoing phase II clinical trials. In vitro, Irvalec induces a rapid loss of membrane integrity in tumor cells, accompanied of a significant Ca2+ influx, perturbations of membrane conductivity, severe swelling and the formation of giant membranous vesicles. All these effects are not observed in Irvalec-resistant cells, or are significantly delayed by pretreating the cells with Zn2+. Using fluorescent derivatives of Irvalec it was demonstrated that the compound rapidly interacts with the plasma membrane of tumor cells promoting lipid bilayer restructuration. Also, FRET experiments demonstrated that Irvalec molecules localize in the cell membrane close enough to each other as to suggest that the compound could self-organize, forming supramolecular structures that likely trigger cell death by necrosis through the disruption of membrane integrity. eng Attribution-NonCommercial-NoDerivs 3.0 Unported Irvalec inserts into the plasma membrane causing rapid loss of integrity and necrotic cell death in tumor cells info:eu-repo/semantics/article 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URL https://uvadoc.uva.es/bitstream/10324/45067/1/Irvalec-inserts-into-plasma.pdf File MD5 db7384ec9179caa566225e630fb8d1db 4199150 application/pdf Irvalec-inserts-into-plasma.pdf