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oai:uvadoc.uva.es:10324/450752021-06-24T07:17:05Zcom_10324_44585com_10324_954com_10324_894col_10324_44586

Mitochondrial [Ca2+] oscillations driven by local high [Ca2+] domains generated by spontaneous electric activity Villalobos Jorge, Carlos Núñez Llorente, Lucía Chamero, Pablo Alonso Alonso, María Teresa García-Sancho Martín, Francisco Javier Producción Científica Mitochondria take up calcium during cell activation thus shaping Ca2+ signaling and exocytosis. In turn, Ca2+ uptake by mitochondria increases respiration and ATP synthesis. Targeted aequorins are excellent Ca2+ probes for subcellular analysis, but single-cell imaging has proven difficult. Here we combine virus-based expression of targeted aequorins with photon-counting imaging to resolve dynamics of the cytosolic, mitochondrial, and nuclear Ca2+ signals at the single-cell level in anterior pituitary cells. These cells exhibit spontaneous electric activity and cytosolic Ca2+oscillations that are responsible for basal secretion of pituitary hormones and are modulated by hypophysiotrophic factors. Aequorin reported spontaneous [Ca2+] oscillations in all the three compartments, bulk cytosol, nucleus, and mitochondria. Interestingly, a fraction of mitochondria underwent much larger [Ca2+] oscillations, which were driven by local high [Ca2+] domains generated by the spontaneous electric activity. These oscillations were large enough to stimulate respiration, providing the basis for local tune-up of mitochondrial function by the Ca2+ signal. 2021-01-19T12:23:20Z 2021-01-19T12:23:20Z 2001 info:eu-repo/semantics/article Journal of Biological Chemistry, 2001, vol. 276, n. 43. p. 40293-40297 0021-9258 http://uvadoc.uva.es/handle/10324/45075 10.1074/jbc.C100465200 eng https://www.sciencedirect.com/science/article/pii/S0021925820601107 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/3.0/ © 2001 Elsevier Attribution-NonCommercial-NoDerivs 3.0 Unported Elsevier SI