2026-04-27T08:19:29Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/450782021-06-24T07:17:13Zcom_10324_44585com_10324_954com_10324_894col_10324_44586

Ordóñez Morán, Paloma Larriba, María Jesús García Palmer, Héctor Valero, Ruth Ana Barbáchano Becerril, Antonio Duñach Masjuan, Mireia García de Herreros, Antonio Villalobos Jorge, Carlos Berciano, María Teresa Lafarga Coscojuela, Miguel Ángel Muñoz Terol, Alberto 2008 Producción Científica The active vitamin D metabolite 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) inhibits proliferation and promotes differentiation of colon cancer cells through the activation of vitamin D receptor (VDR), a transcription factor of the nuclear receptor superfamily. Additionally, 1,25(OH)2D3 has several nongenomic effects of uncertain relevance. We show that 1,25(OH)2D3 induces a transcription-independent Ca2+ influx and activation of RhoA–Rho-associated coiled kinase (ROCK). This requires VDR and is followed by activation of the p38 mitogen-activated protein kinase (p38MAPK) and mitogen- and stress-activated kinase 1 (MSK1). As shown by the use of chemical inhibitors, dominant-negative mutants and small interfering RNA, RhoA–ROCK, and p38MAPK-MSK1 activation is necessary for the induction of CDH1/E-cadherin, CYP24, and other genes and of an adhesive phenotype by 1,25(OH)2D3. RhoA–ROCK and MSK1 are also required for the inhibition of Wnt–β-catenin pathway and cell proliferation. Thus, the action of 1,25(OH)2D3 on colon carcinoma cells depends on the dual action of VDR as a transcription factor and a nongenomic activator of RhoA–ROCK and p38MAPK-MSK1. application/pdf http://uvadoc.uva.es/handle/10324/45078 eng Rockefeller University Press RhoA–ROCK and p38MAPK-MSK1 mediate vitamin D effects on gene expression, phenotype, and Wnt pathway in colon cancer cells info:eu-repo/semantics/article TEXT UVaDOC. Repositorio Documental de la Universidad de Valladolid Hispana