2026-04-30T05:05:53Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/450952026-03-16T12:49:14Zcom_10324_44580com_10324_954com_10324_894col_10324_44581

Lorenzo Martín, Elvira Gallego Muñoz, Patricia Ibares Frías, Lucía Marcos, Susana Pérez Merino, Pablo Fernández Martínez, Itziar Kochevar, Irene E Martínez García, María del Carmen 2021-01-21T13:23:07Z 2021-01-21T13:23:07Z 2018 Investigative Ophthalmology & Visual Science, 2018, vol. 59. p. 4821-4830 1552-5783 http://uvadoc.uva.es/handle/10324/45095 10.1167/iovs.18-24881 Producción Científica Purpose: To study corneal wound healing after two cross-linking techniques using either rose bengal and green light (RGX) or the conventional treatment using riboflavin and UVA radiation (UVX). Methods: Corneas of New Zealand rabbits were monolaterally treated with UVX (21 eyes) or RGX (25 eyes). Treatments involved corneal de-epithelialization (8-mm diameter), soaking with photosensitizer (0.1% riboflavin in 20% dextran for 30 minutes for UVX; 0.1% rose bengal for 2 minutes for RGX), and light irradiation (370 nm, 3 mW/cm2, 30 minutes for UVX; 532 nm, 0.25 W/cm2, 7 minutes for RGX). Contralateral eyes were used as controls. Clinical follow-up included fluorescein staining, haze measurement, and pachymetry. Healing events analyzed after euthanasia at 2, 30, and 60 days included cell death (TUNEL assay), cell proliferation (BrdU [bromodeoxyuridine] immunofluorescence), and differentiation to myofibroblasts (α-SMA [alpha smooth muscle actin] immunohistochemistry). Results: Re-epithelialization and pachymetries were similar after RGX and UVX. The haze from day 1 to 15 was greater after UVX. Cell death was deeper after UVX, being localized in the anterior and middle stroma, and was superficial (anterior third) after RGX. Cell proliferation appeared after 2 days and was localized in the middle and posterior stroma in the UVX group but was superficial in the RGX group. After 60 days the number of stromal cells had not returned to the control number in either group. Conclusions: The deeper and longer-lasting cell damage caused by UVX compared to RGX may underlie the slower cell repopulation after UVX and other differences in healing. Shallower damage and a shorter treatment time suggest that RGX may be appropriate for stiffening thin corneas. Consejo Europeo de Investigación (grant ERC-2011-AdC-294099) Gobierno de España (grants FIS2011-25637-R, FIS2014- 56643-R and FIS2017-84753-R) application/pdf eng Association for Research in Vision and Ophthalmology info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ © 2018 Association for Research in Vision and Ophthalmology Attribution-NonCommercial-NoDerivatives 4.0 Internacional Wound repair Cicatrización Cross-linking technique Técnica de cross-linking Rose bengal Rosa de Bengala Ultraviolet radiation Radiación ultravioleta Cornea Córnea 2209.15 Optometría Rose bengal and green light versus riboflavin–UVA cross-linking: Corneal wound repair response info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://iovs.arvojournals.org/article.aspx?articleid=2705917 SI