2026-04-22T21:01:43Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/452532025-09-17T07:50:28Zcom_10324_40357com_10324_952com_10324_894col_10324_40358

Fernández Martínez, Itziar López Miguel, Alberto Enriquez De Salamanca Aladro, Amalia Teson Yudego, María Luisa Stern, Michael E. González García, María Jesús Calonge, Margarita Producción Científica Purpose:To investigate response profiles in the lacrimal functional unit of dry eye disease (DED) and healthy volunteers after exposure to a controlled adverse desiccating environment (CADE) by identifying groups of individuals with similar clinical and molecular changes. Methods:Clinical parameters and tear molecule levels of 20 mild-moderate DED patients and 20 healthy volunteers were evaluated pre- (baseline) and post-CADE exposure. Clustering based on relative change from baseline values was used to identify response profiles. One-vs-all logistic regression was used to identify baseline predictors for response clusters. Results: Four response profiles were identified. Cluster 1: tear break-up time (TBUT) decrease and matrix metalloproteinase 9 (MMP-9) increase. Cluster 2: marked increase in corneal staining, up-regulation of both MMP-9 and interleukin (IL)-6 levels, and down-regulation of epithelial growth factor (EGF). Cluster 3: increase in fractalkine, vascular endothelial growth factor (VEGF), MMP-9, IL-6, IL-8, IL-1 receptor antagonist (IL-1Ra) and RANTES (regulated on activation, normal T expressed and secreted) tear levels; and increased corneal staining and decreased TBUT and phenol red thread scores. Cluster 4: decreased single-item score dry eye questionnaire (SIDEQ) scores and increased corneal staining. Predictive models using baseline variables found that cluster membership depended on: corneal and conjunctival staining, SIDEQ score, interferon gamma-induced protein (IP)-10, VEGF, and IL-1Ra concentrations. Conclusions:The response of both mild-moderate DED and healthy asymptomatic individuals to environmental stress (CADE) can be predicted based on baseline (pre-exposure) clinical and tear molecular parameters. Thus, identifying individuals with a predictable response could improve patient enrollment in DED clinical trials. 2021-02-11 2021-02-11 2019 info:eu-repo/semantics/article Ocul Surf, 2019 Jul;17(3):502-515. http://uvadoc.uva.es/handle/10324/45253 10.1016/j.jtos.2019.03.009 502 515 Response profiles to a controlled adverse desiccating environment based on clinical and tear molecule changes 17 eng https://www.sciencedirect.com/science/article/pii/S1542012418304622 info:eu-repo/semantics/openAccess © 2019 Elsevier Elsevier