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oai:uvadoc.uva.es:10324/46202021-10-21T14:38:02Zcom_10324_1132com_10324_931com_10324_894col_10324_1205

Martín, C. Alonso Revuelta, María Isabel Santiago, C. Moro Balbás, José Antonio Mano Bonín, Anibal de la Carretero Soto, Raquel Gato Casado, Ángel Luis Tubo neural Embriología Neurogénesis Producción Científica Particularly evCerebrospinal fluid has shown itself to be an essential brain component during development. This is parident at the earliest stages of development where a lot of research, performed mainly in chick embryos, supports the evidence that cerebrospinal fluid is involved in different mechanisms controlling brain growth and morphogenesis, by exerting a trophic effect on neuroepithelial precursor cells (NPC) involved in controlling the behaviour of these cells. Despite it being known that cerebrospinal fluid in mammals is directly involved in corticogenesis at fetal stages, the influence of cerebrospinal fluid on the activity of NPC at the earliest stages of brain development has not been demonstrated. Here, using ‘‘in vitro’’ organotypic cultures of rat embryo brain neuroepithelium in order to expose NPC to or deprive them of cerebrospinal fluid, we show that the neuroepithelium needs the trophic influence of cerebrospinal fluid to undergo normal rates of cell survival, replication and neurogenesis, suggesting that NPC are not self-sufficient to induce their normal activity. This data shows that cerebrospinal fluid is an essential component in chick and rat early brain development, suggesting that its influence could be constant in higher vertebrates. 2014-04-22 2014-04-22 2009 info:eu-repo/semantics/article International Journal of Developmental Neuroscience, 2009, vol. 27. p. 733-740 0736-5748 http://uvadoc.uva.es/handle/10324/4620 733 27 740 Internatioanl Journal Developmental Neuroscience 27 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ Elsevier Attribution-NonCommercial-NoDerivatives 4.0 International Elsevier