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oai:uvadoc.uva.es:10324/466372022-07-18T09:33:08Zcom_10324_35294com_10324_952com_10324_894col_10324_35295

Paniagua García, Ana I. Hijosa Valsero, María Garita Cambronero, Jerson Coca Sanz, Mónica Díez Antolínez, Rebeca 2021-05-25T09:27:24Z 2021-05-25T09:27:24Z 2019 Microchemical Journal, 2019, vol. 150, p. 104147 0026-265X https://uvadoc.uva.es/handle/10324/46637 10.1016/j.microc.2019.104147 104147 Microchemical Journal 150 Producción Científica Lignocellulosic agro-food wastes are regarded as interesting carbohydrate sources for acetone-butanol-ethanol (ABE) fermentation. However, the physicochemical and enzymatic pretreatments applied to release their sugars generate inhibitory compounds that hinder the fermentation. The release of inhibitory compounds in the hydrolysates of four agro-food industrial wastes [apple pomace (AP), potato peel (PP), brewers' spent grain (BSG) and coffee silverskin (CS)] obtained after various chemical pretreatments (acid, alkali, organic solvents and surfactant pretreatments) was analyzed. Sixty-seven potential inhibitors were identified by gas chromatography–mass spectrometry (GC–MS) and were classified into non-aromatic compounds (aliphatic acids, nitrogen-containing compounds, furans and fatty acids) and aromatic compounds (phenolics and non-phenolics). Then, a high performance liquid chromatography method with diode array detection (HPLC-DAD) was developed and validated for the quantification of the main potential inhibitors identified in the hydrolysates (i.e. gallic, 3,4-dihydroxybenzoic, 2,5-dihydroxibenzoic, 4-hydroxybenzoic, 3-hydroxybenzoic, vanillic, caffeic, syringic, p-coumaric, and ferulic acids, vanillin, syringaldehyde and caffeine). The proposed HPLC-DAD method was simple, fast and robust and allowed the direct injection of samples without previous treatment, enabling the simultaneous quantification of the abovementioned compounds for the first time. The method was successfully applied to the analysis of AP, PP, BSG and CS hydrolysates. Instituto Nacional de Investigaciones Agronómicas (INIA) (DOC-INIA, grant number DOC 2013-010) H2020-LCE-2015 Waste2Fuels project (Sustainable production of next generation biofuels from waste streams - Waste2Fuels. GA - 654623) application/pdf eng Elsevier info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ © 2019 Elsevier Attribution-NonCommercial-NoDerivatives 4.0 Internacional Residuos industriales agroalimentarios Compuesto fenólico Hidrolizado 23 Química Development and validation of a HPLC-DAD method for simultaneous determination of main potential ABE fermentation inhibitors identified in agro-food waste hydrolysates info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion https://www.sciencedirect.com/science/article/pii/S0026265X1930877X info:eu-repo/grantAgreement/EC/H2020/654623 SI