2026-05-05T11:17:19Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/484992025-03-04T12:41:08Zcom_10324_22821com_10324_954com_10324_894col_10324_22822

00925njm 22002777a 4500 dc Guillot-Ferriols, Maria author Barrio, Ana del author Costa, Carlos M. author Lanceros Méndez, Senentxu author Rodríguez Cabello, José Carlos author Gómez Ribelles, José Luis author Santos García, María Mercedes author Gallego Ferrer, Gloria author 2021 Bone’s inherent piezoelectricity is a key factor in regulating bone growth and mesenchymal stem cells (MSCs) fate towards the osteogenic lineage. The piezoelectric polymer poly(vinylidene) fluoride (PVDF) was thus used to manufacture electroactive membranes by means of non-solvent induced phase separation (NIPS), producing porous membranes with approximately 90% of γ-phase for MSCs culture. The combination of the porous surface and PVDF hydrophobicity hinders cell adhesion and requires a coating to improve cell culture conditions. A layer-by-layer (LbL) method was used to deposit elastin-like recombinamers (ELRs) containing RGD sequences applying click cross-linking chemistry. ELRs potential was confirmed by comparing traditional fibronectin adsorption with ELRs LbL on PVDF electroactive membranes. Porcine bone marrow MSCs preferred ELRs-coated surfaces, which enhanced initial cell adhesion and improved proliferation after 7 days. These findings lead to new possibilities for regenerative therapies in the area of bone tissue engineering, offering the advantages of MSC commitment towards the osteogenic lineage by applying electro-mechanical stimulation on electroactive substrates. European Polymer Journal 146 (2021) 110269 Available 0014-3057 https://uvadoc.uva.es/handle/10324/48499 10.1016/j.eurpolymj.2021.110269 110269 146 European Polymer Journal 146 Effective elastin-like recombinamers coating on poly(vinylidene) fluoride membranes for mesenchymal stem cell culture