2026-04-27T13:50:33Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/484992025-03-04T12:41:08Zcom_10324_22821com_10324_954com_10324_894col_10324_22822

Effective elastin-like recombinamers coating on poly(vinylidene) fluoride membranes for mesenchymal stem cell culture Guillot-Ferriols, Maria Barrio, Ana del Costa, Carlos M. Lanceros Méndez, Senentxu Rodríguez Cabello, José Carlos Gómez Ribelles, José Luis Santos García, María Mercedes Gallego Ferrer, Gloria Producción Científica Bone’s inherent piezoelectricity is a key factor in regulating bone growth and mesenchymal stem cells (MSCs) fate towards the osteogenic lineage. The piezoelectric polymer poly(vinylidene) fluoride (PVDF) was thus used to manufacture electroactive membranes by means of non-solvent induced phase separation (NIPS), producing porous membranes with approximately 90% of γ-phase for MSCs culture. The combination of the porous surface and PVDF hydrophobicity hinders cell adhesion and requires a coating to improve cell culture conditions. A layer-by-layer (LbL) method was used to deposit elastin-like recombinamers (ELRs) containing RGD sequences applying click cross-linking chemistry. ELRs potential was confirmed by comparing traditional fibronectin adsorption with ELRs LbL on PVDF electroactive membranes. Porcine bone marrow MSCs preferred ELRs-coated surfaces, which enhanced initial cell adhesion and improved proliferation after 7 days. These findings lead to new possibilities for regenerative therapies in the area of bone tissue engineering, offering the advantages of MSC commitment towards the osteogenic lineage by applying electro-mechanical stimulation on electroactive substrates. 2021-09-02T11:50:59Z 2021-09-02T11:50:59Z 2021 info:eu-repo/semantics/article European Polymer Journal 146 (2021) 110269 Available 0014-3057 https://uvadoc.uva.es/handle/10324/48499 10.1016/j.eurpolymj.2021.110269 110269 146 European Polymer Journal 146 spa www.elsevier.com/locate/europolj info:eu-repo/semantics/openAccess Elsevier SI