2026-04-28T19:49:15Zhttps://uvadoc.uva.es/oai/request
oai:uvadoc.uva.es:10324/509742021-12-19T19:34:46Zcom_10324_22821com_10324_954com_10324_894col_10324_22822
UVaDOC author Salinas Fernández, Soraya author Santos García, María Mercedes author Alonso Rodrigo, Matilde author Quintanilla Sierra, Luis author Rodríguez Cabello, José Carlos 2021-12-17T12:14:21Z 2021-12-17T12:14:21Z 2020 Applied Materials Today, 2020, vol. 18, 100500 2352-9407 https://uvadoc.uva.es/handle/10324/50974 10.1016/j.apmt.2019.100500 The versatility of 3D bioprinting techniques has demonstrated great potential for the development of artificial engineered tissues that more closely resemble native tissues. Despite this, challenges remain as regards the search for new bioinks that embrace all the complex parameters that this technique demands. In an attempt to develop such an advanced material, a novel smart material based on elastin-like recombinamers (ELRs) has been developed by molecularly programming its sequence to exhibit a sequential three-stage gelation process, thus providing printing attributes. The thermoresponsive behavior of the ELR is exploited for the deposition of well-controlled fibres on a platform heated to 37 °C, and its recombinant nature ensures batch-to-batch reproducibility and its applicability to a desired target tissue by the introduction of selected bioactive amino-acid sequences into its molecular chain. The biocompatible nature of the ELR enables the printing of loaded cells while providing a protective environment as part of the printing process. Thus, HFF-1 cells were found to be able to proliferate within the printed structures upon culture, displaying their natural morphology. The results of this work highlight the applicability and novelty of the bioprinting of biomimetic ELR-based structures for advanced applications. eng Atribución 4.0 Internacional Genetically engineered elastin-like recombinamers with sequence-based molecular stabilization as advanced bioinks for 3D bioprinting info:eu-repo/semantics/article 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URL https://uvadoc.uva.es/bitstream/10324/50974/1/Genetically-engineered-elastin-like-recombinamers.pdf File MD5 a78c797e10a7e2c3dd8a17d42522f2b8 4344527 application/pdf Genetically-engineered-elastin-like-recombinamers.pdf