2026-04-14T19:19:54Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/511492024-12-18T08:40:26Zcom_10324_1179com_10324_931com_10324_894col_10324_1306

00925njm 22002777a 4500 dc Vega, L. de author Pérez Fernández, Rebeca author Martin Mateo, María Concepción author Bustamante Bustamante, Jesús author Mendiluce Herrero, Alicia author Bustamante Munguira, Elena author 2002 The aim of this work is to study the temporary variation of oxidative stress in renal transplants, both in plasma andin erythrocytes (CR). In order to do so, we determined total glutathione (GST) levels, both oxidized (GSSG) and reduced (GSH), and the activity of enzymes, glutathione peroxidase (G-px), glutathione reductase (G-red) and glutathione transferase (GSt), in renal transplant patients. Determinations were made 48 h before the transplant 1 week and 2 weeks after the renal transplant. The results obtainedconfirm a high ‘‘oxidative stress’’ rate, resulting from the equilibrium between the production of free radicals andthe activity of antioxidants, the former being higher proportionally. Immediately after the transplant there is an increase of oxidative stress, which results in an increase of G-red, a marked decrease of G-px in plasma andin erythrocytes (CR) andan abrupt drop both in GST levels in plasma andin GSG (as well as in the [GSH]/[GSSG] relationship). As times goes on, after the transplant, there is a significant improvement in the activity of antioxidant enzymes, but there is no normalization, which is easily seen in the fact that total glutathione levels andthe activity of the various enzymes approach the average values of the control group. Renal Failure, 2002, vol. 24, n. 4, p. 421–432 0886-022X https://uvadoc.uva.es/handle/10324/51149 10.1081/JDI-120006769 421 4 432 Renal Failure 24 1525-6049 Riñones - Trasplante Riñones - Enfermedades Enzimas Glutathione determination and a study of the activity of glutathione-peroxidase, glutathione-transferase, and glutathione-reductase in renal transplants