2026-05-05T20:04:43Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/550632022-09-14T06:17:18Zcom_10324_22821com_10324_954com_10324_894col_10324_22822

Hasan, Abshar Bagnol, Romain Owen, Robert Latif, Arsalan Rostam, Hassan M. Elsharkawy, Sherif Rose, Felicity R. A. J. Rodríguez Cabello, José Carlos Ghaemmaghami, Amir M. Eglin, David Mata, Álvaro Cirugía maxilofacial Producción Científica Design and fabrication of implants that can perform better than autologous bone grafts remain an unmet challenge for the hard tissue regeneration in craniomaxillofacial applications. Here, we report an integrated approach combining additive manufacturing with supramolecular chemistry to develop acellular mineralizing 3D printed scaffolds for hard tissue regeneration. Our approach relies on an elastin-like recombinamer (ELR) coating designed to trigger and guide the growth of ordered apatite on the surface of 3D printed nylon scaffolds. Three test samples including a) uncoated nylon scaffolds (referred to as “Uncoated”), b) ELR coated scaffolds (referred to as “ELR only”), and c) ELR coated and in vitro mineralized scaffolds (referred to as “Pre- mineralized”) were prepared and tested for in vitro and in vivo performance. All test samples supported normal human immortalized mesenchymal stem cell adhesion, growth, and differentiation with enhanced cell proliferation observed in the “Pre- mineralized” samples. Using a rabbit calvarial in vivo model, ‘Pre-mineralized’ scaffolds also exhibited higher bone ingrowth into scaffold pores and cavities with higher tissue- implant integration. However, the coated scaffolds (“ELR only” and “Pre-mineralized”) did not exhibit significantly more new bone formation compared to “Uncoated” scaffolds. Overall, the mineralizing coating offers an opportunity to enhance integration of 3D printed bone implants. However, there is a need to further decipher and tune their immunologic response to develop truly osteoinductive/conductive surfaces. 2022-09-13 2022-09-13 2022 info:eu-repo/semantics/article Frontiers in Bioengineering and Biotechnology, 2022, vol. 10, artículo 836386 Frontiers in Bioengineering and Biotechnology, 2022, vol. 10, artículo 836386 2296-4185 https://uvadoc.uva.es/handle/10324/55063 10.3389/fbioe.2022.836386 1 12 Frontiers in Bioengineering and Biotechnology 10 2296-4185 eng https://www.frontiersin.org/articles/10.3389/fbioe.2022.836386/full info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ © The author(s) © The author(s) Attribution-NonCommercial-NoDerivatives 4.0 Internacional Frontiers Media