2026-05-05T19:53:03Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/550902023-03-15T21:00:24Zcom_10324_22821com_10324_954com_10324_894col_10324_22822

González Obeso, Constancio Rodríguez Cabello, José Carlos Kaplan, David L. 2022-09-14T07:14:15Z 2022-09-14T07:14:15Z 2022 Acta Biomaterialia, 2022, vol. 141, p. 14-23 1878-7568 https://uvadoc.uva.es/handle/10324/55090 10.1016/j.actbio.2021.12.031 Producción Científica Elastin-like polymers (ELPs) and their chimeric subfamily the silk elastin-like polymers (SELPs) exhibit a lower critical solvation temperature (LCST) behavior in water which has been extensively studied from theoretical, computational and experimental perspectives. The inclusion of silk domains in the backbone of the ELPs effects the molecular dynamics of the elastin-like domains in response to increased temperature above its transition temperature and confers gelation ability. This response has been studied in terms of initial and long-term changes in structures, however, intermediate transition states have been less investigated. Moreover, little is known about the effects of reversible hydration on the elastin versus silk domains in the physical crosslinks. We used spectroscopic techniques to analyze initial, intermediate and long-term states of the crosslinks in SELPs. A combination of thermoanalytical and rheological measurements demonstrated that the fast reversible rehydration of the elastin motifs adjacent to the relatively small silk domains was capable of breaking the silk physical crosslinks. This feature can be exploited to tailor the dynamics of these types of crosslinks in SELPs. National Institutes of Health (grant P41EB027062) ARO (grant W911NF-17-1-0384) Gobierno de España (grants PID2019-110709RB-100 and RED2018-102417-T) Junta de Castilla y León (grants VA317P18 and Infrared2018-UVA06) Interreg V España Portugal POCTEP (grant 0624_2IQBIONEURO_6_E) application/pdf eng Elsevier info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ © 2021 Elsevier Attribution-NonCommercial-NoDerivatives 4.0 Internacional Polymers Polímeros Elastin Elastina Crosslinking Reticulación Fast and reversible crosslinking of a silk elastin-like polymer info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion https://www.sciencedirect.com/science/article/pii/S174270612100845X?via%3Dihub SI