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A novel correlation for rapid lactose determination in milk by a cryoscopic technique Colinas González, María del Carmen Barrera, Ignacio Blanco Fuentes, Carlos Antonio Leche - Análisis Alimentos - Composición Alimentos - Calidad - Control 3309 Tecnología de Los Alimentos Producción Científica Residual lactose in special milk was systematically determined for people with lactose intolerance by means of a rapid on-line measurement of the cryoscopic point. A proposed cryoscopic procedure was compared to 2 conventional yet highly laborious methods: the enzymatic procedure with spectrophotometric control and the polarimetric method. Several experiments with different mixtures of both semi-skimmed and low-lactose milk were performed. A lineal relationship was found between lactose concentration and freezing point, the analytical equation for which shows a close relationship regarding the 3 methods used. The advantages of the cryoscopic procedure include speed in obtaining results and operational simplicity at a low cost, better monitoring of enzymatic hydrolysis kinetics, and greater control over the production process for delactosed milk. The equation obtained also enables prediction of the lactose percentage in commercial milk by a simple measurement of freezing point. 2022-09-22T11:20:19Z 2022-09-22T11:20:19Z 2006 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Journal of AOAC International, Vol. 89, Nº 6, 2006, págs. 1581-1584 1060-3271 https://uvadoc.uva.es/handle/10324/55572 10.1093/jaoac/89.6.1581 1581 6 1584 Journal of AOAC International 89 1944-7922 eng https://academic.oup.com/jaoac/article/89/6/1581/5657760 Attribution-NonCommercial-NoDerivatives 4.0 Internacional info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ © Oxford University Press © AOAC International application/pdf Oxford University Press https://uvadoc.uva.es/bitstream/10324/55572/4/A-novel-correlation-for-rapid-lactose-determination-in-milk.pdf.jpg Hispana TEXT http://creativecommons.org/licenses/by-nc-nd/4.0/ UVaDOC. Repositorio Documental de la Universidad de Valladolid https://uvadoc.uva.es/handle/10324/55572