2026-04-27T19:45:55Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/557592026-02-24T08:05:46Zcom_10324_32522com_10324_952com_10324_894col_10324_32523

Guijas Maté, Carlos Bermúdez Arias, Miguel Ángel Meana González, Clara Astudillo del Valle, Alma María Pereira, Laura Fernández Caballero, Lidia Balboa García, María Ángeles Balsinde Rodríguez, Jesús 2022-10-03T11:38:26Z 2022-10-03T11:38:26Z 2019 Cells 2019, vol. 8, n. 8, 941 0214-4842 https://uvadoc.uva.es/handle/10324/55759 10.3390/cells8080941 Producción Científica Human monocytes exposed to free arachidonic acid (AA), a secretory product of endothelial cells, acquire a foamy phenotype which is due to the accumulation of cytoplasmic lipid droplets with high AA content. Recruitment of foamy monocytes to the inflamed endothelium contributes to the development of atherosclerotic lesions. In this work, we investigated the potential role of AA stored in the neutral lipids of foamy monocytes to be cleaved by lipases and contribute to lipid mediator signaling. To this end, we used mass spectrometry-based lipidomic approaches combined with strategies to generate monocytes with different concentrations of AA. Results from our experiments indicate that the phospholipid AA pool in monocytes is stable and does not change upon exposure of the cells to the external AA. On the contrary, the AA pool in triacylglycerol is expandable and can accommodate relatively large amounts of fatty acid. Stimulation of the cells with opsonized zymosan results in the expected decreases of cellular AA. Under all conditions examined, all of the AA decreases observed in stimulated cells were accounted for by decreases in the phospholipid pool; we failed to detect any contribution of the triacylglycerol pool to the response. Experiments utilizing selective inhibitors of phospholipid or triacylglyerol hydrolysis confirmed that the phospholipid pool is the sole contributor of the AA liberated by stimulated cells. Thus, the AA in the triacylglycerol is not a source of free AA for the lipid mediator signaling during stimulation of human foamy monocytes and may be used for other cellular functions. Ministerio de Economía, Industria y Competitividad (project SAF2016-80883-R) application/pdf eng MDPI info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ © 2019 The Authors Atribución 4.0 Internacional Arachidonic acid Ácido araquidónico Mass spectrometry Espectrometría de masas Neutral lipids are not a source of arachidonic acid for lipid mediator signaling in human foamy monocytes info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.mdpi.com/2073-4409/8/8/941 SI