2026-04-22T22:18:48Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/564652025-01-10T13:48:34Zcom_10324_1134com_10324_931com_10324_894col_10324_1213

Gallego Martín, María Teresa Prieto Lloret, Jesús Aaronson, Philip Irving Rocher Martín, María Asunción Obeso Cáceres, Ana María de la Luz 2022-10-24T09:41:33Z 2022-10-24T09:41:33Z 2019 Antioxidants, 2019, vol. 8, n. 3, 62 2076-3921 https://uvadoc.uva.es/handle/10324/56465 10.3390/antiox8030062 Producción Científica Carotid body (CB) chemoreceptor cells sense arterial blood PO2, generating a neurosecretory response proportional to the intensity of hypoxia. Hydrogen sulfide (H2S) is a physiological gaseous messenger that is proposed to act as an oxygen sensor in CBs, although this concept remains controversial. In the present study we have used the H2S scavenger and vitamin B12 analog hydroxycobalamin (Cbl) as a new tool to investigate the involvement of endogenous H2S in CB oxygen sensing. We observed that the slow-release sulfide donor GYY4137 elicited catecholamine release from isolated whole carotid bodies, and that Cbl prevented this response. Cbl also abolished the rise in [Ca2+]i evoked by 50 µM NaHS in enzymatically dispersed CB glomus cells. Moreover, Cbl markedly inhibited the catecholamine release and [Ca2+]i rise caused by hypoxia in isolated CBs and dispersed glomus cells, respectively, whereas it did not alter these responses when they were evoked by high [K+]e. The L-type Ca2+ channel blocker nifedipine slightly inhibited the rise in CB chemoreceptor cells [Ca2+]i elicited by sulfide, whilst causing a somewhat larger attenuation of the hypoxia-induced Ca2+ signal. We conclude that Cbl is a useful and specific tool for studying the function of H2S in cells. Based on its effects on the CB chemoreceptor cells we propose that endogenous H2S is an amplifier of the hypoxic transduction cascade which acts mainly by stimulating non-L-type Ca2+ channels. Ministerio de Economía, Industria y Competitividad - Fondo Europeo de Desarrollo Regional (project BFU2015-70616-R) Junta de Castilla y León (project VA106G18) Centro de Investigación Biomédica en Red de Enfermedades Respiratorias . Instituto de Salud Carlos III (project CIBER CB06/06/0050) application/pdf eng MDPI info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ © 2019 The Authors Atribución 4.0 Internacional Carotid body Cuerpo carotídeo Hydrogen sulfide Ácido sulfhídrico Hypoxia Hipoxia Hydroxycobalamin Hidroxocobalamina Hydroxycobalamin reveals the involvement of hydrogen sulfide in the hypoxic responses of rat carotid body chemoreceptor cells info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.mdpi.com/2076-3921/8/3/62 SI