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Unlocking the high-rate continuous performance of fermentative hydrogen bioproduction from fruit and vegetable residues by modulating hydraulic retention time Martínez Mendoza, Leonardo José García Depraect, Octavio Muñoz Torre, Raúl Biohydrogen Biorefinery Fruit-vegetable waste Dark fermentation Biohidrógeno Biorrefinería Fermentación oscura Residuos de frutas y verduras 3302.02 Tecnología de la Fermentación Producción Científica Harnessing fruit-vegetable waste (FVW) as a resource to produce hydrogen via dark fermentation (DF) embraces the circular economy concept. However, there is still a need to upgrade continuous FVW-DF bioprocessing to enhance hydrogen production rates (HPR). This study aims to investigate the influence of the hydraulic retention time (HRT) on the DF of FVW by mixed culture. A stirred tank reactor under continuous mesophilic conditions was operated for 47 days with HRT stepwise reductions from 24 to 6 h, leading to organic loading rates between 47 and 188 g volatile solids (VS)/L-d. The optimum HRT of 9 h resulted in an unprecedented HPR from FVW of 11.8 NL/L-d, with a hydrogen yield of 95.6 NmL/g VS fed. Based on an overarching inspection of hydrogen production in conjunction with organic acids and carbohydrates analyses, it was hypothesized that the high FVW-to-biohydrogen conversion rate achieved was powered by lactate metabolism. European Commission-H2020-MSCA-IF-2019 project UP-GRAD (894515) Junta de Castilla y Leon - FEDER (program CLU 2017-09, CL-EI-2021-07 & UIC 315) 2023-02-16T09:55:04Z 2023-02-16T09:55:04Z 2023 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Bioresource Technology, Volume 373, 2023, 128716 0960-8524 https://uvadoc.uva.es/handle/10324/58680 10.1016/j.biortech.2023.128716 128716 Bioresource Technology 373 eng https://www.sciencedirect.com/science/article/pii/S0960852423001426?via%3Dihub info:eu-repo/grantAgreement/EC/H2020/894515 Attribution-NonCommercial-NoDerivatives 4.0 Internacional info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ © 2023 The Authors application/pdf Elsevier https://uvadoc.uva.es/bitstream/10324/58680/4/Unlocking-high-rate.pdf.jpg Hispana TEXT http://creativecommons.org/licenses/by-nc-nd/4.0/ UVaDOC. Repositorio Documental de la Universidad de Valladolid https://uvadoc.uva.es/handle/10324/58680