2026-04-14T21:07:21Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/586902025-03-04T08:56:20Zcom_10324_28542com_10324_952com_10324_894col_10324_28543

Ares Sacristán, Ana María Toribio Recio, Laura García Villalba, Rocío Villalgordo, Jose M. Althobaiti, Yusuf Tomás Barberán, Francisco A. Bernal del Nozal, José 2023 Producción Científica Urolithins are gut microbiota metabolites produced in humans after consuming foods containing ellagitannins and ellagic acid. Three urolithin metabotypes have been reported for different individuals depending on the final urolithins produced. After absorption, they are conjugated with glucuronic acid (phase II metabolism), and these are the main circulating metabolites in plasma and reach different tissues. Different regioisomeric isomers of urolithin glucuronides have been described. Still, their identification and quantification in humans have not been properly reported due to resolution limitations in their analysis by reversed-phase high-performance liquid chromatography. In the present study, we report a novel method for separating these isomers using supercritical fluid chromatography. With this method, urolithin A 3- and 8-glucuronide, isourolithin A 3- and 9- glucuronide, and urolithin B 3-glucuronide (8-hydroxy urolithin 3-glucuronide; 3-hydroxy urolithin 8-glucuronide; 3-hydroxyurolithin 9-glucuronide; 9-hydroxyurolithin 3-glucuronide; and urolithin 3-glucuronide) were separated in less than 15 min. The proposed method was applied to successfully analyze these metabolites in urine samples from different volunteers belonging to different metabotypes. application/pdf https://uvadoc.uva.es/handle/10324/58690 eng American Chemical Society Química analítica 2301 Química Analítica Separation of isomeric forms of urolithin glucuronides using supercritical fluid chromatography info:eu-repo/semantics/article TEXT UVaDOC. Repositorio Documental de la Universidad de Valladolid Hispana