2026-04-28T19:26:49Zhttps://uvadoc.uva.es/oai/request
oai:uvadoc.uva.es:10324/589682023-03-17T20:02:43Zcom_10324_1134com_10324_931com_10324_894col_10324_1213
UVaDOC author Iglesias Álvarez, María del Rosario author Polito, Letizia author Bortolotti, Massimo author Pedrazzi, Manuela author Citores González, Lucía author Ferreras Rodríguez, José Miguel author Bolognesi, Andrea 2023-03-17T09:12:41Z 2023-03-17T09:12:41Z 2020 Toxins, 2020, Vol. 12, Nº. 9, 538 2072-6651 https://uvadoc.uva.es/handle/10324/58968 10.3390/toxins12090538 538 9 Toxins 12 2072-6651 Stenodactylin is one of the most potent type 2 ribosome-inactivating proteins (RIPs); its high toxicity has been demonstrated in several models both in vitro and in vivo. Due to its peculiarities, stenodactylin could have several medical and biotechnological applications in neuroscience and cancer treatment. In this work, we report the complete amino acid sequence of stenodactylin and 3D structure prediction. The comparison between the primary sequence of stenodactylin and other RIPs allowed us to identify homologies/differences and the amino acids involved in RIP toxic activity. Stenodactylin RNA was isolated from plant caudex, reverse transcribed through PCR and the cDNA was amplificated and cloned into a plasmid vector and further analyzed by sequencing. Nucleotide sequence analysis showed that stenodactylin A and B chains contain 251 and 258 amino acids, respectively. The key amino acids of the active site described for ricin and most other RIPs are also conserved in the stenodactylin A chain. Stenodactylin amino acid sequence shows a high identity degree with volkensin (81.7% for A chain, 90.3% for B chain), whilst when compared with other type 2 RIPs the identity degree ranges from 27.7 to 33.0% for the A chain and from 42.1 to 47.7% for the B chain. eng Atribución 4.0 Internacional Proteins - Synthesis Proteínas Ribosomes - Structure Primary sequence and 3D structure prediction of the plant toxin stenodactylin info:eu-repo/semantics/article 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URL https://uvadoc.uva.es/bitstream/10324/58968/1/Primary-Sequence-and-3D%20Structure-Prediction-of-the-Plant-Toxin-Stenodactylin%20.pdf File MD5 7d149165ffb2f65b152d0e99bc44cf1a 7726923 application/pdf Primary-Sequence-and-3D Structure-Prediction-of-the-Plant-Toxin-Stenodactylin .pdf