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oai:uvadoc.uva.es:10324/590642025-09-23T12:54:27Zcom_10324_35294com_10324_952com_10324_894col_10324_35295

Rojo De Benito, Elena María Filipigh , Ángel Alejandro Bolado Rodríguez, Silvia 2023-04-10T08:44:56Z 2023-04-10T08:44:56Z 2023 Process Safety and Environmental Protection, 2023, vol. 174, p. 276-285 0957-5820 https://uvadoc.uva.es/handle/10324/59064 10.1016/j.psep.2023.03.067 Process Safety and Environmental Protection Producción Científica Despite the interest in the utilization of photobioreactors as an alternative wastewater treatment, the research about posterior recovery and valorization of nutrients accumulated in the biomass is still limited. This work compared several hydrolysis methods for the recovery of proteins and carbohydrates from the biomass grown in a photobioreactor treating swine wastewater. Ultrasound-assisted and microwave-assisted enzymatic hydrolysis at mild conditions and chemical methods at different temperatures (40, 60, 120ºC) were applied to the microalgae and bacteria biomass. Alkaline hydrolysis provided the greatest peptide recoveries, increasing with temperature up to a maximum of 81%, but with very small peptide sizes in all temperature range. Acid hydrolysis provided the highest carbohydrate recoveries (60.7% at 120ºC) but degraded proteins, even at mild temperatures. Protein degradation did not vary with temperature in each chemical hydrolysis, obtaining similar peptide sizes in all temperatures, while carbohydrate losses were higher at lower temperatures. Ultrasound-assisted enzymatic extraction recovered 43.6% of the initial proteins as large peptides (up to 135 kDa) with the highest peptide purity (46.7%). Microwave-assistance increased the carbohydrate solubilization of enzymatic hydrolysis, achieving yields of 73% of xylose, but with significant losses. Ministerio de Ciencia e Innovación y Ministerio de Universidades (PID2020-113544RB-I00 y PDC2021-121861-C22) Junta de Castilla y León (UIC 338, CLU 2017-09) UE-FEDER (CLU 2017-09) application/pdf eng Elsevier info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ © 2023 The Authors Attribution-NonCommercial-NoDerivatives 4.0 Internacional Aguas residuales Depuración Biomasa Ultrasounds Proteins Peptides Piggery wastewater Ultrasonidos Proteínas Péptidos Aguas residuales de pocilga 3303 Ingeniería y Tecnología Químicas 3308 Ingeniería y Tecnología del Medio Ambiente Assisted-enzymatic hydrolysis vs chemical hydrolysis for fractional valorization of microalgae biomass info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0957582023002719?via%3Dihub SI