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Impact of optimal timing of intake of multi-ingredient performance supplements on sports performance, muscular damage, and hormonal behavior across a ten-week training camp in elite cyclists: A randomized clinical trial Fernández Lázaro, Diego Mielgo Ayuso, Juan Francisco Valle Soto, Miguel del Adams, David P. Gutiérrez Abejón, Eduardo Seco Calvo, Jesús Nutrición Deporte Supplementation Amino acids Muscle recovery Sport performance Suplementación Aminoácidos Recuperación muscular Rendimiento deportivo 2407 Biología Celular 3206.08 Nutrientes Producción Científica Multi-ingredient performance supplements (MIPS), ingested pre- or post-workout, have been shown to increase physiological level effects and integrated metabolic response on exercise. The purpose of this study was to determine the efficacy of pre-and post-training supplementation with its own MIPS, associated with CHO (1 g·kg−1) plus protein (0.3 g·kg−1) on exercise-related benchmarks across a training camp for elite cyclists. Thirty elite male cyclists participated in a randomized non-placebo-controlled trial for ten weeks assigned to one of three groups (n = 10 each): a control group treated with CHO plus protein after training (CG); a group treated with MIPS before training and a CHO plus protein after training, (PRE-MIPS); a group treated with CHO plus protein plus MIPS after training, (POST-MIPS). Performance parameters included (VO2max, peak; median and minimum power (W) and fatigue index (%)); hormonal response (Cortisol; Testosterone; and Testosterone/Cortisol ratio); and muscle biomarkers (Creatine kinase (CK), Lactate dehydrogenase (LDH), and Myoglobin (Mb)) were assessed. MIPS administered before or after training (p ≤ 0.05) was significantly influential in attenuating CK, LDH, and MB; stimulating T response and modulating C; and improved on all markers of exercise performance. These responses were greater when MIPS was administered post-workout. 2023-05-03T09:15:47Z 2023-05-03T09:15:47Z 2021 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Nutrients, 2021, vol. 13, n. 11, 3746 https://uvadoc.uva.es/handle/10324/59466 10.3390/nu13113746 3746 11 Nutrients 13 2072-6643 eng https://www.mdpi.com/2072-6643/13/11/3746 Atribución 4.0 Internacional info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ © 2021 The Authors application/pdf MDPI https://uvadoc.uva.es/bitstream/10324/59466/4/Impact-of-Optimal-Timing-of-Intake.pdf.jpg Hispana TEXT http://creativecommons.org/licenses/by/4.0/ UVaDOC. Repositorio Documental de la Universidad de Valladolid https://uvadoc.uva.es/handle/10324/59466