2026-04-26T07:15:44Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/594982023-05-05T19:00:53Zcom_10324_40357com_10324_952com_10324_894col_10324_40358

Cañadas Suárez, María del Pilar Lantigua Dorville, Yrbani Enriquez De Salamanca Aladro, Amalia Fernández Martínez, Itziar Pastor Idoate, Salvador Sobas Abad, Eva María Dueñas Laita, Antonio Pérez Castrillon, José Luis Pastor Jimeno, José Carlos Calonge, Margarita Ophthalmology Ojos - Enfermedades Eye - Diseases Cornea - Diseases - Diagnosis Confocal microscopy Cytokines Dry eye syndromes Producción Científica Purpose: To report the ocular surface pathology of patients suffering from acute/subacute mercury vapor intoxication. Design: Cross-sectional study. Participants: Male workers intoxicated with inorganic mercury referred for ophthalmic involvement and healthy control subjects. Methods: The following tests were performed: dry eye (DE)-related symptoms indicated by the ocular surface disease (OSDI) index questionnaire; tear osmolarity; analysis of 23 tear cytokine concentrations and principal component and hierarchical agglomerative cluster analyses; tear break-up time (T-BUT); corneal fluorescein and conjunctival lissamine green staining; tear production by Schirmer and tear lysozyme tests; mechanical and thermal corneal sensitivity (non-contact esthesiometry); and corneal nerve analysis and dendritic cell density by in vivo confocal microscopy (IVCM). Results: Twenty-two out of 29 evaluated patients entered the study. Most had DE-related symptoms (OSDI values > 12), that were severe in 63.6% of them. Tear osmolarity was elevated (>308 mOsms/L) in 83.4% of patients (mean 336.23 (28.71) mOsm/L). Corneal and conjunctival staining were unremarkable. T-BUT was low (<7 s) in 22.7% of patients. Schirmer test and tear lysozyme concentration were low in 13.6% and 27.3% of cases, respectively. Corneal esthesiometry showed patient mechanical (mean 147.81 (53.36) mL/min) and thermal thresholds to heat (+2.35 (+1.10) °C) and cold (−2.57 (−1.24) °C) to be significantly higher than controls. Corneal IVCM revealed lower values for nerve density (6.4 (2.94) n/mm2), nerve branching density (2 (2.50) n/mm2), and dendritic cell density (9.1 (8.84) n/mm2) in patients. Tear levels of IL-12p70, IL-6, RANTES, and VEGF were increased, whereas EGF and IP-10/CXCL10 were decreased compared to controls. Based on cytokine levels, two clusters of patients were identified. Compared to Cluster 1, Cluster 2 patients had significantly increased tear levels of 18 cytokines, decreased tear lysozyme, lower nerve branching density, fewer dendritic cells, and higher urine mercury levels. Conclusions: Patients suffering from systemic mercury intoxication showed symptoms and signs of ocular surface pathology, mainly by targeting the trigeminal nerve, as shown by alterations in corneal sensitivity and sub-basal nerve morphology. 2023-05-05 2023-05-05 2021 info:eu-repo/semantics/article Diagnostics, 2021, Vol. 11, Nº. 8, 1326 2075-4418 https://uvadoc.uva.es/handle/10324/59498 10.3390/diagnostics11081326 1326 8 Diagnostics 11 2075-4418 eng https://www.mdpi.com/2075-4418/11/8/1326 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ © 2021 The authors Atribución 4.0 Internacional MDPI