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oai:uvadoc.uva.es:10324/60992025-03-03T10:24:07Zcom_10324_1134com_10324_931com_10324_894col_10324_1213

Apolipoprotein D alters the early transcriptional response to oxidative stress in the adult cerebellum Bajo Grañeras, Raquel Sánchez Romero, Diego Gutiérrez, Gabriel González Martínez, Constancio Carmo, Sonia do Rassart, Eric Ganfornina Álvarez, María Dolores Nervioso, sistema - Fisiología Producción Científica The lipocalin Apolipoprotein D (ApoD), known to protect the nervous system against oxidative stress (OS) in model organisms, is up-regulated early in the mouse brain in response to the ROS generator paraquat. However, the processes triggered by this up-regulation have not been explored. We present here a study of the effect of ApoD on the early transcriptional changes upon OS in the mouse cerebellum using microarray profiling. ApoD-KO and transgenic mice over-expressing ApoD in neurons are compared to wild-type controls. In control conditions, ApoD affects the transcriptional profile of neuron and oligodendrocyte-specific genes involved in neuronal excitability, synaptic function, and myelin homeostasis. When challenged with paraquat, the absence of ApoD modifies the response of genes mainly related to OS management and myelination. Interestingly, the over-expression of ApoD in neurons almost completely abolishes the early transcriptional response to OS. We independently evaluate the expression of protein kinase Cd, a gene up-regulated by OS only in the ApoD-KO cerebellum, and find it overexpressed in cultured ApoD-KO primary astrocytes, which points to a role for ApoD in astrocyte-microglia signaling. Our results support the hypothesis that ApoD is necessary for a proper response of the nervous system against physiological and pathological OS. 2015-09-19 2014-09-19T16:34:44Z 2015-09-19T23:40:08Z 2011 info:eu-repo/semantics/article Journal of Neurochemistry, 2011, vol. 117, p. 949-960 0022-3042 http://uvadoc.uva.es/handle/10324/6099 10.1111/j.1471-4159.2011.07266.x 949 960 Journal of Neurochemistry 117 eng Attribution-NonCommercial-NoDerivatives 4.0 International info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf International Society for Neurochemistry https://uvadoc.uva.es/bitstream/10324/6099/6/S%c3%81NCHEZ%2013%5b1%5d.pdf.jpg Hispana TEXT http://creativecommons.org/licenses/by-nc-nd/4.0/ UVaDOC. Repositorio Documental de la Universidad de Valladolid http://uvadoc.uva.es/handle/10324/6099