2026-05-05T20:03:38Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/62732021-06-23T09:48:36Zcom_10324_1133com_10324_931com_10324_894col_10324_1209

Differential expression and localization of transient receptor potential vanilloid 1 in rabbit and human eyes Martínez García, María del Carmen Martínez Valcárcel, Tamara Pañeda Vázquez-Prada, Covadonga Gallego Muñoz, Patricia Jimenez, Ana I. Merayo Lloves, Jesús Cornea - Enfermedades - Tratamiento Producción Científica Summary. Introduction: The superfamily of transient receptor potential (TRP) cation channels is involved in nociception. Members of this family, such as the vanilloid receptor type 1 (TRPV1) channel, are activated by a wide range of stimuli including heat (>43°C), low pH (<6.5), hypoxia, and hypertonicity. Here we report TRPV1 expression in rabbit and human eyes. Material and methods: We analyzed the expression of TRPV1 mRNA by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and protein by immunohistochemistry in eyes of New Zealand White rabbits and humans. Results: In rabbit and human eyes, TRPV1 protein was present in all layers of the corneal epithelium, but only in the basal layer of the conjunctiva. It was also in the ciliary and lens epithelia of both species as well as in the secretory cells of the rabbit lacrimal gland. The retinal pigment epithelium was positive for this protein in both species. TRPV1 was also present in rabbit Müller cells, where it had a similar pattern of expression to vimentin intermediate filaments. Analysis by qRT-PCR showed that TRPV1 mRNA was found in all of the structures where the protein was present. The highest level was in the lens and the lowest in the retina. Conclusion: TRPV1 is expressed in cells that are particularly active in Ca2+ exchange as well as in cells with significant water transport activity. Because TRPV1 is a Ca2+ channel, it probably functions in the regulation of both water and Ca2+ movements in ocular tissues. 2014-09-25T17:05:10Z 2014-09-25T17:05:10Z 2013 info:eu-repo/semantics/article Histology and Histopatology, 2013, vol. 28, p. 1507-1516 0213-3911 http://uvadoc.uva.es/handle/10324/6273 1507 1516 Histology and Histopatology 28 eng Attribution-NonCommercial-NoDerivatives 4.0 International info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Universidad de Murcia https://uvadoc.uva.es/bitstream/10324/6273/6/MARTINEZ-%20GARCIA%203.pdf.jpg Hispana TEXT http://creativecommons.org/licenses/by-nc-nd/4.0/ UVaDOC. Repositorio Documental de la Universidad de Valladolid http://uvadoc.uva.es/handle/10324/6273