2026-04-27T08:22:58Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/634792025-02-21T12:33:29Zcom_10324_35294com_10324_952com_10324_894col_10324_35295
Santos Beneit, Fernando
Chen, Le Min
Bordel Velasco, Sergio
Frutos de la Flor, Raquel
García Depraect, Octavio
Lebrero Fernández, Raquel
Rodriguez Vega, Sara
Muñoz Torre, Raúl
Börner, Rosa Aragão
Börner, Tim
2023-12-05T08:22:17Z
2023-12-05T08:22:17Z
2023
Microorganisms, 2023, Vol. 11, Nº. 2, 328
2076-2607
https://uvadoc.uva.es/handle/10324/63479
10.3390/microorganisms11020328
328
2
Microorganisms
11
2076-2607
Producción Científica
In recent years, a number of microbial enzymes capable of degrading plastics have been identified. Biocatalytic depolymerization mediated by enzymes has emerged as a potentially more efficient and environmentally friendly alternative to the currently employed methods for plastic treatment and recycling. However, the functional and systematic study of depolymerase enzymes with respect to the degradation of a series of plastic polymers in a single work has not been widely addressed at present. In this study, the ability of a set of enzymes (esterase, arylesterase and cutinase) to degrade commercial biodegradable polymers (PBS, PBAT, PHB, PHBH, PHBV, PCL, PLA and PLA/PCL) and the effect of pre-treatment methods on their degradation rate was assessed. The degradation products were identified and quantified by HPLC and LC-HRMS analysis. Out of the three enzymes, Fusarium solani cutinase (FsCut) showed the highest activity on grinded PBAT, PBS and PCL after 7 days of incubation. FsCut was engineered and heterologous expressed in Escherichia coli, which conferred the bacterium the capability of degrading solid discs of PBAT and to grow in PBS as the sole carbon source of the medium.
Société des Produits Nestlé S.A, Junta de Castilla y León y Fondo Europeo de Desarrollo Regional (FEDER) - (grant CL-EI-2021-07 and UIC 315)
application/pdf
eng
MDPI
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
© 2023 The authors
Atribución 4.0 Internacional
Biodegradable plastics
Polymeric composites
Depolymerization
Polymers
Polymerization
Polimeros y polimerizacion
Plastics
Materias plásticas
Sustainable development
Desarrollo sostenible
Renewable natural resources
Recursos naturales renovables
Enzymes
Enzimas
Microbiology
Environment
2206.10 Polímeros
2302.09 Enzimología
2414 Microbiología
3308 Ingeniería y Tecnología del Medio Ambiente
Screening enzymes that can depolymerize commercial biodegradable polymers: Heterologous expression of Fusarium solani cutinase in Escherichia coli
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
https://www.mdpi.com/2076-2607/11/2/328
SI