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oai:uvadoc.uva.es:10324/63482021-06-23T09:51:57Zcom_10324_1134com_10324_931com_10324_894col_10324_1213

The coupling of plasma membrane calcium entry to calcium uptake by endoplasmic reticulum and mitochondria García-Sancho Martín, Francisco Javier Calcio - Absorción Producción Científica Cross-talk between organelles and plasma membrane Ca2+ channels is essential for modulation of the cytosolic Ca2+ ([Ca2+]C) signals, but such modulation may differ among cells. In chromaffin cells Ca2+ entry through voltage-operated channels induces calcium release from the endoplasmic reticulum (ER) that amplifies the signal. [Ca2+]C microdomains as high as 20–50 μM are sensed by subplasmalemmal mitochondria, which accumulate large amounts of Ca2+ through the mitochondrial Ca2+ uniporter (MCU). Mitochondria confine the high-Ca2+ microdomains (HCMDs) to beneath the plasma membrane,where exocytosis of secretory vesicles happens. Cell core [Ca2+]C is much smaller (1–2 μM). By acting as a Ca2+ sink, mitochondria stabilise theHCMDin space and time. In non-excitableHEK293 cells, activation of store-operated Ca2+ entry, triggered by ERCa2+ emptying, also generated subplasmalemmal HCMDs, but, in this case, most of the Ca2+ was taken up by the ER rather than bymitochondria. The smaller size of the [Ca2+]C peak in this case (about 2 μM)may contribute to this outcome, as the sarco-endoplasmic reticulum Ca2+ ATPase has much higher Ca2+ affinity than MCU. It is also possible that the relative positioning of organelles, channels and effectors, as well as cytoskeleton and accessory proteins plays an important role. 2014-09-30T17:17:39Z 2015-09-30T23:40:08Z 2014 info:eu-repo/semantics/article Journal of Physiology, 2014, p. 261-268 0022-3751 http://uvadoc.uva.es/handle/10324/6348 10.1113/jphysiol.2013.255661 261 268 Journal of Physiology eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ Attribution-NonCommercial-NoDerivatives 4.0 International The Physiological Society SI