2026-04-28T21:12:21Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/636002025-09-09T10:09:09Zcom_10324_35294com_10324_952com_10324_894col_10324_35295
Martín González, Diego
Bordel Velasco, Sergio
Solis, Selvin
Gutierrez Merino, Jorge
Santos Beneit, Fernando
2023-12-13T12:17:04Z
2023-12-13T12:17:04Z
2023
Microorganisms, 2023, Vol. 11, Nº. 2, 456
2076-2607
https://uvadoc.uva.es/handle/10324/63600
10.3390/microorganisms11020456
456
2
Microorganisms
11
2076-2607
Two efficient feather-degrading bacteria were isolated from honeybee samples and identified as Bacillus sonorensis and Bacillus licheniformis based on 16S rRNA and genome sequencing. The strains were able to grow on chicken feathers as the sole carbon and nitrogen sources and degraded the feathers in a few days. The highest keratinase activity was detected by the B. licheniformis CG1 strain (3800 U × mL−1), followed by B. sonorensis AB7 (1450 U × mL−1). Keratinase from B. licheniformis CG1 was shown to be active across a wide range of pH, potentially making this strain advantageous for further industrial applications. All isolates displayed antimicrobial activity against Micrococcus luteus; however, only B. licheniformis CG1 was able to inhibit the growth of Mycobacterium smegmatis. In silico analysis using BAGEL and antiSMASH identified gene clusters associated with the synthesis of non-ribosomal peptide synthetases (NRPS), polyketide synthases (PKSs) and/or ribosomally synthesized and post-translationally modified peptides (RiPPs) in most of the Bacillus isolates. B. licheniformis CG1, the only strain that inhibited the growth of the mycobacterial strain, contained sequences with 100% similarity to lichenysin (also present in the other isolates) and lichenicidin (only present in the CG1 strain). Both compounds have been described to display antimicrobial activity against distinct bacteria. In summary, in this work, we have isolated a strain (B. licheniformis CG1) with promising potential for use in different industrial applications, including animal nutrition, leather processing, detergent formulation and feather degradation.
eng
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
© 2023 The authors
Atribución 4.0 Internacional
Bacillus (Bacteria)
Honey
Miel
Bees
Abejas
Keratin
Proteins
Antibiotics
Feeds
Biodegradation
Biodegradación
Microorganisms
Microorganismos
Microbiology
Characterization of Bacillus strains from natural honeybee products with high keratinolytic activity and antimicrobial potential
info:eu-repo/semantics/article