2026-04-14T18:07:56Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/637292025-01-10T13:52:54Zcom_10324_1134com_10324_931com_10324_894col_10324_1213

Guillem Marti, Jordi Vidal, Elia Girotti ., Alessandra Heras Parets, Aina Torres, Diego Arias Vallejo, Francisco Javier Ginebra, M. P. Rodríguez Cabello, José Carlos Manero, José Maria 2023-12-20T08:41:03Z 2023-12-20T08:41:03Z 2023 Pharmaceutics, 2023, Vol. 15, Nº. 3, 872 1999-4923 https://uvadoc.uva.es/handle/10324/63729 10.3390/pharmaceutics15030872 872 3 Pharmaceutics 15 2073-4360 1999-4923 The 3D printing of titanium (Ti) offers countless possibilities for the development of personalized implants with suitable mechanical properties for different medical applications. However, the poor bioactivity of Ti is still a challenge that needs to be addressed to promote scaffold osseointegration. The aim of the present study was to functionalize Ti scaffolds with genetically modified elastin-like recombinamers (ELRs), synthetic polymeric proteins containing the elastin epitopes responsible for their mechanical properties and for promoting mesenchymal stem cell (MSC) recruitment, proliferation, and differentiation to ultimately increase scaffold osseointegration. To this end, ELRs containing specific cell-adhesive (RGD) and/or osteoinductive (SNA15) moieties were covalently attached to Ti scaffolds. Cell adhesion, proliferation, and colonization were enhanced on those scaffolds functionalized with RGD-ELR, while differentiation was promoted on those with SNA15-ELR. The combination of both RGD and SNA15 into the same ELR stimulated cell adhesion, proliferation, and differentiation, although at lower levels than those for every single moiety. These results suggest that biofunctionalization with SNA15-ELRs could modulate the cellular response to improve the osseointegration of Ti implants. Further investigation on the amount and distribution of RGD and SNA15 moieties in ELRs could improve cell adhesion, proliferation, and differentiation compared to the present study. eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ © 2023 The authors Atribución 4.0 Internacional 3D Printing Three-dimensional printing Impresión tridimensional Imágenes tridimensionales en medicina Diseño asistido por ordenador Polymers in medicine Polímeros recombinantes Polímeros - Aplicaciones médicas Biomaterials Biomateriales Osseointegration Titanium Pharmaceutical technology Pharmacology Functionalization of 3D-printed titanium scaffolds with elastin-like recombinamers to improve cell colonization and osteoinduction info:eu-repo/semantics/article