2026-04-26T21:58:41Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/643042024-12-16T09:02:03Zcom_10324_1134com_10324_931com_10324_894col_10324_1213

Wei, Qianru Brzostek, Joanna Sankaran, Shvetha Casas Requena, Javier Hew, Lois Shi-Qi Yap, Jiawei Zhao, Xiang Wojciech, Lukasz Gascoigne, Nicholas R. J. 2020 Producción Científica Src family kinase Lck plays critical roles during T cell development and activation, as it phosphorylates the TCR/CD3 complex to initiate TCR signaling. Lck is present either in coreceptor-bound or coreceptor-unbound (free) forms, and we here present evidence that the two pools of Lck have different molecular properties. We discovered that the free Lck fraction exhibited higher mobility than CD8α-bound Lck in OT-I T hybridoma cells. The free Lck pool showed more activating Y394 phosphorylation than the coreceptor-bound Lck pool. Consistent with this, free Lck also had higher kinase activity, and free Lck mediated higher T cell activation as compared to coreceptor-bound Lck. Furthermore, the coreceptor-Lck coupling was independent of TCR activation. These findings give insights into the initiation of TCR signaling, suggesting that changes in coreceptor-Lck coupling constitute a mechanism for regulation of T cell sensitivity. application/pdf https://uvadoc.uva.es/handle/10324/64304 eng Lck bound to coreceptor is less active than free Lck info:eu-repo/semantics/article TEXT UVaDOC. Repositorio Documental de la Universidad de Valladolid Hispana