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2024-01-30T11:49:37Z urn:hdl:10324/65304 Development of a novel in vitro model to study the modulatory role of the respiratory complex I in macrophage effector functions Serrano-Lorenzo, Pablo Gobelli, Dino Garrido-Moraga, Rocío Esteban Amo, María Jesús Orduña, Antonio Fuente García, Miguel Ángel de la Martín, Miguel A. López López, José Ramón Simarro Grande, María Increasing evidence demonstrate that the electron transfer chain plays a critical role in controlling the effector functions of macrophages. In this work, we have generated a Ndufs4−/− murine macrophage cell lines. The Ndufs4 gene, which encodes a supernumerary subunit of complex I, is a mutational hotspot in Leigh syndrome patients. Ndufs4−/− macrophages showed decreased complex I activity, altered complex I assembly, and lower levels of maximal respiration and ATP production. These mitochondrial respiration alterations were associated with a shift towards a pro-inflammatory cytokine profile after lipopolysaccharide challenge and improved ability to phagocytose Gram-negative bacteria. 2024-01-30T11:49:37Z 2024-01-30T11:49:37Z 2023 info:eu-repo/semantics/article PLoS ONE, 18(9 September), 1–15. https://uvadoc.uva.es/handle/10324/65304 10.1371/journal.pone.0291442 e0291442 9 PLOS ONE 18 1932-6203 spa info:eu-repo/semantics/openAccess