2026-04-28T19:36:45Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/653042024-12-13T13:29:42Zcom_10324_43677com_10324_954com_10324_894col_10324_43678
Development of a novel in vitro model to study the modulatory role of the respiratory complex I in macrophage effector functions
Serrano-Lorenzo, Pablo
Gobelli, Dino
Garrido-Moraga, Rocío
Esteban Amo, María Jesús
Orduña, Antonio
Fuente García, Miguel Ángel de la
Martín, Miguel A.
López López, José Ramón
Simarro Grande, María
Increasing evidence demonstrate that the electron transfer chain plays a critical role in controlling the effector functions of macrophages. In this work, we have generated a Ndufs4−/− murine macrophage cell lines. The Ndufs4 gene, which encodes a supernumerary subunit of complex I, is a mutational hotspot in Leigh syndrome patients. Ndufs4−/− macrophages showed decreased complex I activity, altered complex I assembly, and lower levels of maximal respiration and ATP production. These mitochondrial respiration alterations were associated with a shift towards a pro-inflammatory cytokine profile after lipopolysaccharide challenge and improved ability to phagocytose Gram-negative bacteria.
2024-01-30
2024-01-30
2023
info:eu-repo/semantics/article
PLoS ONE, 18(9 September), 1–15.
https://uvadoc.uva.es/handle/10324/65304
10.1371/journal.pone.0291442
e0291442
9
PLOS ONE
18
1932-6203
spa
info:eu-repo/semantics/openAccess