2026-05-05T11:44:11Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/653042024-12-13T13:29:42Zcom_10324_43677com_10324_954com_10324_894col_10324_43678

00925njm 22002777a 4500 dc Serrano-Lorenzo, Pablo author Gobelli, Dino author Garrido-Moraga, Rocío author Esteban Amo, María Jesús author Orduña, Antonio author Fuente García, Miguel Ángel de la author Martín, Miguel A. author López López, José Ramón author Simarro Grande, María author 2023 Increasing evidence demonstrate that the electron transfer chain plays a critical role in controlling the effector functions of macrophages. In this work, we have generated a Ndufs4−/− murine macrophage cell lines. The Ndufs4 gene, which encodes a supernumerary subunit of complex I, is a mutational hotspot in Leigh syndrome patients. Ndufs4−/− macrophages showed decreased complex I activity, altered complex I assembly, and lower levels of maximal respiration and ATP production. These mitochondrial respiration alterations were associated with a shift towards a pro-inflammatory cytokine profile after lipopolysaccharide challenge and improved ability to phagocytose Gram-negative bacteria. PLoS ONE, 18(9 September), 1–15. https://uvadoc.uva.es/handle/10324/65304 10.1371/journal.pone.0291442 e0291442 9 PLOS ONE 18 1932-6203 Development of a novel in vitro model to study the modulatory role of the respiratory complex I in macrophage effector functions