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oai:uvadoc.uva.es:10324/654472024-01-31T20:02:10Zcom_10324_65189com_10324_954com_10324_894col_10324_65190

Citores González, Lucía Iglesias Álvarez, María del Rosario Ragucci, Sara Di Maro, Antimo Ferreras Rodríguez, José Miguel 2024-01-31T12:52:55Z 2024-01-31T12:52:55Z 2018 ACS Chemical Biology, Agosto 2018, vol. 13 (8), p. 1978-1982 1554-8929 https://uvadoc.uva.es/handle/10324/65447 10.1021/acschembio.8b00410 1978 8 1982 ACS Chemical Biology 13 1554-8937 Producción Científica Among the putative defense proteins that occur in fungi, one of the best studied is α-sarcin, produced by the mold Aspergillus giganteus. This protein is the most significant member of the ribotoxin family, which consists of extracellular rRNA ribonucleases that display cytotoxic activity toward animal cells. Ribotoxins are rRNA endonucleases that catalyze the hydrolysis of the phosphodiester bond between G4325 and A4326 from the rat 28S rRNA. The results of several experimental approaches have led to propose ribotoxins as insecticidal agents. In this work, we report that α-sarcin displays a strong antifungal activity against Penicillium digitatum, being able to enter into the cytosol where it inactivates the ribosomes, thus killing the cells and arresting the growth of the fungus. This is the first time that a ribotoxin has been found to display antifungal activity. Therefore, this protein could play, besides the already proposed insecticidal function, a role in nature as an antifungal agent. Consejería de Sanidad de la Junta de Castilla y León Regional Grant BIO/VA39/14 application/pdf eng American Chemical Society info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ Copyright © 2018 American Chemical Society Attribution-NonCommercial-NoDerivatives 4.0 Internacional Attribution-NonCommercial-NoDerivatives 4.0 Internacional ribotoxin green mould antifungal agent apoptosis Ribosome-inactivating protein alpha-sarcin Penicillium digitatum 2302.21 Biología Molecular 2302.27 Proteínas 3108.05 Hongos 3214 Toxicología 2302 Bioquímica Antifungal Activity of α-Sarcin against Penicillium digitatum: Proposal of a New Role for Fungal Ribotoxins info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion https://pubs.acs.org/doi/10.1021/acschembio.8b00410 SI