2026-04-22T22:14:30Zhttps://uvadoc.uva.es/oai/request
oai:uvadoc.uva.es:10324/657382024-12-11T08:18:01Zcom_10324_1131com_10324_931com_10324_894col_10324_1201
UVaDOC author Diez-Valcarce, Marta author Kovač, Katarina author Cook, Nigel author Rodríguez-Lázaro, David author Hernández Pérez, Marta 2024-02-05T14:45:56Z 2024-02-05T14:45:56Z 2011 M Diez-Valcarce, K Kovač, N Cook, D Rodríguez-Lázaro, M Hernández. Construction and analytical application of internal amplification controls (IAC) for detection of food supply chain-relevant viruses by real-time PCR-based assays. Food Analytical Methods 2011, 4, 437-445. 1936-9751 https://uvadoc.uva.es/handle/10324/65738 10.1007/S12161-011-9224-2 437 3 445 Food Analytical Methods 4 1936-976X Se construyeron controles internos de amplificación (CAI) para incorporarlos a ensayos de amplificación de ácidos nucleicos en tiempo real con poliomavirus bovino, virus de la hepatitis A, virus de la hepatitis E, adenovirus humano, norovirus humano del genogrupo I, norovirus humano del genogrupo II, norovirus murino y adenovirus porcino. La adición de cantidades optimizadas de CAI en los ensayos no afectó a los límites de detección de cada virus diana específico. Se simuló una extracción deficiente de ácidos nucleicos virales y se demostró la eficacia de las CAI en la identificación de ensayos fallidos. Las CAI construidas en este estudio pueden utilizarse de forma fiable en sus ensayos específicos para proporcionar un control robusto que pueda aplicarse de forma rutinaria en el análisis de alimentos en busca de virus. spa Construction and Analytical Application of Internal Amplification Controls (IAC) for Detection of Food Supply Chain-Relevant Viruses by Real-Time PCR-Based Assays info:eu-repo/semantics/article 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URL https://uvadoc.uva.es/bitstream/10324/65738/1/27%20Diez-Valcarce%20et%20al%202011%20FAM%20IAC.pdf File MD5 baadc4a44cf4316ae8003e15c24e52d1 642469 application/pdf 27 Diez-Valcarce et al 2011 FAM IAC.pdf