2026-05-05T21:53:06Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/658642025-03-03T10:10:44Zcom_10324_1145com_10324_931com_10324_894col_10324_1254

00925njm 22002777a 4500 dc Ferrer, E. author Peinado, V. I. author Castaneda, J. author Prieto Lloret, Jesús author Olea Fraile, Elena author Gonzalez-Martin, M. C. author Vega Agapito, María Victoria author Diez, M. author Dominguez-Fandos, D. author Obeso Cáceres, Ana María de la Luz author González Martínez, Constancio author Barbera, J. A. author 2011 Cigarette smoke (CS) and chronic hypoxia (CH) can produce pulmonary hypertension. Similarities and differences between both exposures and their interaction have not been explored. The aim of the present study was to investigate the effects of CS and CH, as single factors or in combination, on the pulmonary circulation in the guinea pig. 51 guinea pigs were exposed to CS for 12 weeks and 32 were sham-exposed. 50% of the animals in each group were additionally exposed to CH for the final 2 weeks.We measured pulmonary artery pressure (Ppa), and the weight ratio between the right ventricle (RV) and left ventricle plus the septum. Pulmonary artery contractility in response to noradrenaline (NA), endothelium-dependent vasodilatation and distensibility were evaluated in organ bath chambers. The number of small intrapulmonary vessels showing immunoreactivity to smooth muscle (SM) a-actin and double elastic laminas was assessed microscopically. CS and CH induced similar increases of Ppa and RV hypertrophy (p,0.05 for both), effects that were further enhanced when both factors were combined. CH increased the contractility to NA (p,0.01) and reduced the distensibility (p,0.05) of pulmonary arteries. Animals exposed to CS showed an increased number of small vessels with positive immunoreactivity to SM a-actin (p,0.01) and those exposed to CH a greater proportion of vessels with double elastic laminas (p,0.05). We conclude that CH amplifies the detrimental effects of CS on the pulmonary circulation by altering the mechanical properties of pulmonary arteries and enhancing the remodelling of pulmonary arterioles. European Respiratory Journal 0903-1936 https://uvadoc.uva.es/handle/10324/65864 10.1183/09031936.00105110 617 3 627 European Respiratory Journal 38 1399-3003 Effects of cigarette smoke and hypoxia on pulmonary circulation in the guinea pig