2026-04-27T21:26:27Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/670332026-01-22T07:37:13Zcom_10324_40357com_10324_952com_10324_894col_10324_40358

Domínguez López, Alfredo Blanco Vázquez, Marta Calderón García, Andrés Ángel García Vázquez, Carmen González García, María Jesús Calonge, Margarita Enriquez De Salamanca Aladro, Amalia 2024-04-05T09:43:51Z 2024-04-05T09:43:51Z 2024 Experimental Eye Research, abril 2024, vol. 241, 109854 00144835 https://uvadoc.uva.es/handle/10324/67033 10.1016/j.exer.2024.109854 Analysis of the mucosal chemokines CCL28, CXCL14, and CXCL17 in dry eye disease: An in vitro and clinical investigation 241 Producción Científica Mucosal chemokines have antimicrobial properties and play an important role in mucosal immunity. However, little is known about their expression on the ocular surface. This study aimed to analyze the expression of the mucosal chemokines CCL28, CXCL14 and CXCL17 in corneal and conjunctival epithelial cells under in vitro dry eye (DE) conditions, and in conjunctival samples from healthy subjects and DE patients. Human corneal epithelial cells (HCE) and immortalized human conjunctival epithelial cells (IM-ConEpiC) were incubated under hyperosmolar (400–500 mOsM) or inflammatory (TNF-α 25 ng/mL) conditions for 6 h and 24 h to measure CCL28, CXCL14, and CXCL17 gene expression by RT-PCR and their secretion by immunobead-based analysis (CCL28, CXCL14) and ELISA (CXCL17). Additionally, twenty-seven DE patients and 13 healthy subjects were included in this study. DE-related questionnaires (OSDI, mSIDEQ and NRS) evaluated symptomatology. Ocular surface integrity was assessed using vital staining. Tactile sensitivity was measured with Cochet-Bonnet esthesiometer, and mechanic and thermal (heat and cold) sensitivity using Belmonte’s non-contact esthesiometer. Subbasal nerve plexus and dendritic cell density were analyzed by in vivo confocal microscopy. Conjunctival cells from participants were collected by impression cytology to measure mucosal chemokines gene expression by RTPCR. Our results showed that HCE and IM-HConEpiC cells increased CCL28, CXCL14, and CXCL17 secretion under hyperosmolar conditions. The gene expression of CCL28 was significantly upregulated in conjunctival samples from DE patients. CCL28 expression correlated positively with symptomatology, corneal staining, heat sensitivity threshold, and dendritic cell density. CXCL14 expression correlated positively with age, ocular pain, conjunctival staining, tactile sensitivity, and image reflectivity. CXCL17 expression correlated positively with corneal staining. These results suggest that corneal and conjunctival epithelial cells could be a source of CCL28, CXCL14, and CXCL17 on the ocular surface and that CCL28 might be involved in DE pathogenesis. Este trabajo forma parte de los proyectos de investigación: SAF-2016-77080-P, financiado por MICIU/AEI /10.13039/501100011033/ y por FEDER Una manera de hacer Europa; por proyecto PID 2022-142578OB-I00, financiado por MICIU/AEI/10.13039/501100011033 y por FEDER, UE; y por grant SECTEI/160/ 2021 fianciado por Ministerio de Educacion, Ciencia, Tecnología e Innovación de Ciudad de Méjico (SECTEI). application/pdf eng Elsevier info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ © 2024 The Authors Attribution-NonCommercial-NoDerivatives 4.0 Internacional Ocular surface Dry eye Mucosal chemokines Hyperosmolarity Inflammation CCL28 CXCL14 CXCL17 2403 Bioquímica 2410 Biología Humana 2412 Inmunología 2415 Biología Molecular 3299 Otras Especialidades Médicas Analysis of the mucosal chemokines CCL28, CXCL14, and CXCL17 in dry eye disease: An in vitro and clinical investigation info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.sciencedirect.com/science/article/pii/S0014483524000757 SI