2026-04-27T19:49:44Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/691822025-02-19T12:42:08Zcom_10324_22821com_10324_954com_10324_894col_10324_22822

Sarisoy, Alp Acosta Rodríguez, Sergio Rodríguez Cabello, José Carlos Czichowski, Phillip Kopp, Alexander Jockenhoevel, Stefan Fernández Colino, Alicia 2024-07-26T08:43:35Z 2024-07-26T08:43:35Z 2023 International Journal of Molecular Sciences, 2023, Vol. 24, Nº. 7, 6776 1422-0067 https://uvadoc.uva.es/handle/10324/69182 10.3390/ijms24076776 6776 7 International Journal of Molecular Sciences 24 1422-0067 Producción Científica More than 260 million surgical procedures are performed worldwide each year. Although sutures and staples are widely used to reconnect tissues, they can cause further damage and increase the risk of infection. Bioadhesives have been proposed as an alternative to reconnect tissues. However, clinical adhesives that combine strong adhesion with cytocompatibility have yet to be developed. In this study, we explored the production of adhesives based on protein-engineered polymers bioinspired by the sequence of elastin (i.e., elastin-like recombinamers, ELRs). We hypothesized that the combination of polyphenols (i.e., tannic acid, TA) and ELRs would produce an adhesive coacervate (ELR+TA), as reported for other protein polymers such as silk fibroin (SF). Notably, the adhesion of ELR alone surpassed that of ELR+TA. Indeed, ELR alone achieved adhesive strengths of 88.8 ± 33.2 kPa and 17.0 ± 2.0 kPa on porcine bone and skin tissues, respectively. This surprising result led us to explore a multicomponent bioadhesive to encompass the complementary roles of elastin (mimicked here by ELR) and silk fibroin (SF), and subsequently mirror more closely the multicomponent nature of the extracellular matrix. Tensile testing showed that ELR+SF achieved an adhesive strength of 123.3 ± 60.2 kPa on porcine bone and excellent cytocompatibility. To express this in a more visual and intuitive way, a small surface of only 2.5 cm2 was able to lift at least 2 kg of weight. This opens the door for further studies focusing on the ability of protein-engineered polymers to adhere to biological tissues without further chemical modification for applications in tissue engineering. Gobiernos federal y estatal de Alemania - (StUpPD370-20) Ministerio Alemán de Educación e Investigación (BMBF) - (grant 13XP5136) Gobierno de España - (grant PID2019- 110709RB-100, RED2018-102417-T) Junta de Castilla y León - (grant VA317P18, Infrared2018-UVA06) Programa de Cooperación Interreg España-Portugal POCTEP - (grant 0624_2IQBIONEURO_6_E) application/pdf eng MDPI info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ © 2023 The authors Atribución 4.0 Internacional Adhesives Adhesivos Biomaterials Biomateriales Biomedical materials Polymers Proteins Medicine Polímeros recombinantes Tejidos (Histología) Bones Huesos Skin Piel Organic chemistry Inorganic chemistry Molecular biology Medical technology 2206.10 Polímeros 2302.27 Proteínas 2306 Química Orgánica 2303 Química Inorgánica 32 Ciencias Médicas 2415 Biología Molecular 3314 Tecnología Médica Bioglues based on an elastin-like recombinamer: effect of tannic acid as an additive on tissue adhesion and cytocompatibility info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.mdpi.com/1422-0067/24/7/6776 SI