2026-04-28T19:10:15Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/695592025-02-06T11:02:53Zcom_10324_1166com_10324_931com_10324_894col_10324_1338

Sánchez Hernández, Eva Balduque Gil, Joaquín González García, Vicente Barriuso Vargas, Juan José Casanova Gascón, José Martín Gil, Jesús Martín Ramos, Pablo 2024-08-30T08:39:45Z 2024-08-30T08:39:45Z 2023 International Journal of Molecular Sciences, 2023, Vol. 24, Nº. 2, 1154 1422-0067 https://uvadoc.uva.es/handle/10324/69559 10.3390/ijms24021154 1154 2 International Journal of Molecular Sciences 24 1422-0067 Producción Científica Despite extensive research on the chemical composition of elderberries and their numerous uses in pharmaceutical, beverage, and food production, there is still a lack of knowledge about Sambucus nigra leaves and flowers’ antimicrobial activity against plant pathogens. In this study, the phytoconstituents of their aqueous ammonia extracts were first characterized by infrared spectroscopy and gas chromatography–mass spectrometry. The major phytocompounds identified in the flower extract were octyl 2-methylpropanoate; 3,5-dihydroxy-6-methyl-2,3-dihydropyran-4-one; propyl malonic acid; adenine; and 1-methyl-2-piperidinemethanol. Concerning the leaf extract, 1,6-anhydro-β-D-glucopyranose; oleic acid; 2,1,3-benzothiadiazole; 2,3-dihydro-benzofuran; and 4-((1E)-3-hydroxy-1-propenyl)-2-methoxyphenol and other phenol derivatives were the main constituents. The potential of the extracts to act as bioprotectants was then investigated against three almond tree pathogens: Diaporthe amygdali, Phytophthora megasperma, and Verticillium dahliae. In vitro tests showed higher activity of the flower extract, with EC90 values in the 241–984 μg·mL−1 range (depending on the pathogen) vs. 354–1322 μg·mL−1 for the leaf extract. In addition, the flower extract led to full protection against P. megasperma at a dose of 1875 μg·mL−1 in ex situ tests on artificially-infected excised almond stems. These inhibitory concentrations were lower than those of commercial fungicides. These findings suggest that S. nigra aerial organs may be susceptible to valorization as an alternative to synthetic fungicides for the protection of this important crop. Junta de Castilla y León y Fondo Europeo de Desarrollo Regional (FEDER) - (project VA258P18) application/pdf eng MDPI info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ © 2023 The authors Atribución 4.0 Internacional Phytochemicals Fitoquímica Antimicrobial activity Forests and forestry Bosques y silvicultura Plant extracts Almond Almendro Trees - Diseases and pests Arboles - Enfermedades y plagas Pest control Pathogenic fungi Hongos patógenos Fungi Plant science Plant biotechnology Plantas - Biotecnología Plant science 3102 Ingeniería Agrícola 3106 Ciencia Forestal 3106.08 Silvicultura 3108.05 Hongos Phytochemical profiling of Sambucus nigra L. flower and leaf extracts and their antimicrobial potential against almond tree pathogens info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.mdpi.com/1422-0067/24/2/1154 SI