2026-04-14T19:24:54Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/708142025-05-26T07:17:07Zcom_10324_39910com_10324_894col_10324_39911

Amo Mateos, Esther Del Cáceres, Berta Coca Sanz, Mónica García Cubero, María Teresa Lucas Yagüe, Susana Universidad de Valladolid, Instituto de Procesos Sostenibles 2024-10-14T18:28:08Z 2024-10-14T18:28:08Z 2023-09-14 2024 Bioresource Technology, 2024, 394, 130263. https://uvadoc.uva.es/handle/10324/70814 10.71569/ew90-e566 This research focuses on the integrated recovery of rhamnogalacturonan-I (RG-I) pectin from sugar beet pulp (SBP). First, the extraction of RG-I pectin through sequential ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) was assessed. Optimization using a response surface methodology identified the optimal conditions as initial pH 4, 10 minutes of UAE, and 157 °C for MAE, achieving a 66.0 % recovery of pectooligosaccharides (POS). Additionally, purification through continuous diafiltration and concentration via ultrafiltration of the POS using membranes with different molecular weight cut-offs (MWCO) was explored. In contrast to previous research using discontinuous diafiltration, the use of continuous diafiltration allowed a decrease in the extract viscosity and obtained higher yields using a higher MWCO membrane. The refined RG-I pectin solids exhibited a high global yield (39 – 40 g pectin/100 g SBP), and high-methoxyl characteristics, as well as purity levels (70 – 80 %) similar to commercial prebiotics. Instituto de Procesos Sostenibles Spanish Ministry of Science, Innovation and Universities (PID2020-115110RB-I00/AEI/10.13039/501100011033) text/csv eng Estrategias para la valorización efectiva de residuos hortofrutícolas: producción de compuestos bioactivos y biocombustibles avanzados. VALORFRUIT (2021-2024) info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ Attribution-NonCommercial-NoDerivatives 4.0 Internacional Pectooligosaccharides; Box-Behnken design; Green extraction; ultrafiltration; diafiltration Recovering rhamnogalacturonan-I pectin from sugar beet pulp using a sequential ultrasound and microwave-assisted extraction: Study on extraction optimization and membrane purification (dataset) dataset info:eu-repo/semantics/publishedVersion